INJECTIONS OT11KR MASS US (COLD). 267 



iron in 500 c.c. of water is added to a solution of 20 gr. of 

 yellow prussiate of potash in 500 c.c. of water, allowed to 

 stand for twelve hours, decanted, the deposit washed with 

 distilled water on a filter until the washings come through 

 dark blue (one to two days), and the blue dissolved in about 

 a litre of water. It is well to add a little acetic acid and to 

 put up the objects in an acid liquid. 



513. EMERY'S Aqueous Carmine (ibid., 1881, p. 21). To a 10 per 

 cent, ammoiiiacal solution of carmine is added acetic acid, with continual 

 stirring, until the colour of the solution changes to blood-red. The 

 supernatant clear solution is injected cold without further preparation- 

 The injected organs are thrown at once into strong alcohol to fix the 

 carmine. For injection of fishes. 



514. TAGUOHI'S Indian Ink (Arch. mik. Anat., 1888, p. 565). 

 Chinese or (better) Japanese ink well rubbed up on a hone 

 until a fluid is obtained that does not run when dropped on 

 thin blotting-paper, nor form a grey ring round the drop. 

 Inject until the preparation appears quite black, and throw 

 it into some hardening liquid (not pure water). 



DELLA ROSA (Ver. Anat. Ges., 1900, p. 141) recommends 

 the liquid Chinese ink sold in the shops. 



Partially Aqueous Masses. 



515. JOSEPH'S White-of-Egg (Ber. naturw. Sect. Schles. Ges., 

 1879, pp. 8640; Journ. Roy. Mic. Soc., ii, 1882, p. 274). 

 " Filtered white-of-egg, diluted with 1 to 5 per cent, of car- 

 mine solution. . . . This mass remains liquid when cold, 

 coagulates in dilute nitric acid, chromic or osuiic acid, and 

 remains transparent in the vessels." For invertebrates. 



GROSSER (Zeit. wiss. Mik., xvii, 1900, p, 178) rubs up 

 Indian ink with white-of-egg ; HOFFMANN (Zeit. Morph. 

 Anthrop., iii, 1901, p. 240) with blood-serum; so also 

 HAMBURGER, Zeit. wiss. Mik., xxv, 1908, p. 1 (2 vols. of the 

 ink " Perltusche "' to 3 of serum). 



516. BJELOUSSOW'S Gum Arabic Mass (Arch. Anat. Phys., 

 1885, p. 379). Make a syrupy solution of gum arabic and 

 a saturated solution of borax in water. Mix the solutions in 

 such proportions as to have in the mixture 1 part of borax 



