328 ril.MTKU XXVI. 



BUdiogr. Anat., Nov. Gtli, 1897, p. 278, and Joiirn. dt>. I'Anat., 

 xxxvi, 1900, p. 22, and under " Mitochondria," next . 



ALTMANN (Studien fiber die Zelle, 188<>; Die Elementarorganismen 

 Leipzig, 1890 ; Arch. f. Amtt. . KntiM-rl.. 1S92, p. t>^ ; also Zeit. f. 

 wiss. Nik., vii, 2, 1890, p. 199; ix, 3, 1893, p. 331 ; and L. and R. ZOJA, 

 hi Mem. R. 1st. Lombardo di Sci. e Lettere , xvi, 3, vii, p. 237) demon- 

 strates bis " Bioblasts " by fixing for twenty-four hours in a mixture of 

 equal parts of 5 per cent, bichromate of potash and 2 per cent, osmic 

 acid, imbedding in paraffin, staining sections for a minute on the slide 

 held over a flame with a solution of 20 grins, of acid fuchsin in 100 c.c. 

 of aniliii water ( 28(>), and washing out with saturated alcoholic .solution 

 of picric acid diluted with 2 volumes of water, heat being used as before 

 to aid the differentiation, and finally clearing with xylol and UK uniting 

 in balsam. See hereoii the critique of FISCHER, in his Fixininij, 

 Faerbung, u. Ban des Protoplasmas, pp. 108, 295 (these granules 

 mainly artefacts). 



653. Mitochondria (Chondriosom.es, Chondriokonts, Chromidia, 

 Ergastoplasm, etc.). These formations are fixed, more or loss 

 abundantly, by most of the usual fixatives. But some 

 kinds of them seem to be attacked by organic acids ; so 

 that it is well to reduce the proportion of these in mixtures. 

 Thus BEN DA for this purpose, makes up Flemming's strong 

 mixture with only three to six drops of acetic acid to 15 c.c. 

 of the chromic, and 4 c.c. of the osmic. MEVES (Encycl. 

 milt. Techn., 1910, i, p. 476) takes 15 c.c. of chromic acid of 

 0*5 to 1 per cent., containing 1 per cent, of sodium chloride, 

 with 3 to 4 c.c. of osmic acid of 2 per cent., and three to four 

 drops of acetic acid. Similarly, DUESBERG (Arch. Zellf orach. } 

 iv, 1910, p. 605). CHAMPY (Arch. d'Anat. Hie., xiii, 1911, 

 p. 55) takes 7 parts of bichromate of potash of 3 per cent., 

 7 of chromic acid of 1 per cent., and 4 of osmic acid of 2 

 per cent. Or iodide of sodium 15 grms. ; water 800 grins. ; 

 formol 200 grins. ; iodide of mercury to saturation. Or 

 simply formol. Some workers take ALTMANN'S osmic acid and 

 bichromate, 43; so MKVES, Arch. mik. Anat., Ixxvi, 1911, 

 p. 683. 



REGAUD (Arch. Anal, mic., xi, 1910, fasc. 2 and 3) fixes 

 either in 100 parts of 3 per cent, bichromate of potash with 

 20 of formol and 5 of acetic acid ; or 80 parts of the bi- 

 chromate to 20 of formol without acetic acid ; or in 20 parts 

 saturated aqueous picric acid with 5 of formol ; or in formol 

 of 10 per cent. ; and in either case mordants for one to 



