TKCJUMKNTARY OKCUNS. ooo 



(3) Huemalum for two hours, iieutr.il orcein as above f ni- 

 ton to twenty minutes. 



More recently UNNA advocates tlie process mentioned 

 last . 



See alse RANVIER, Arch. Anat. Mikr., iii, 1899, p. 1. 



657. Keratohyalin. The keratohyalin granules of the cells 

 of the stratum gramdosuin are soluble in mineral acids, and 

 can be digested in pepsin. They can be stained with picro- 

 carmine, alum hsematoxylin, van (jieson's mixture, or Unna's 

 Wasserblau-orcein, last . FICK (Centralb. allg. Path., xiii, 

 1902, p. 987 ; Zeit. wiss. Mik., xx, 1903, p. 222) stains sections 

 of alcohol material for three to four minutes in concentrated 

 aqueous solution of Krcsylechtviolett, differentiates in alcohol, 

 clears in xylol, and mounts in balsam. 



See also UNNA, Monatsschr. pralct. Derm., xx, 1895, p. 69 ; the article 

 "Haut" in the Encycl. mile. Technik. ; and UNNA and GOLODETZ, 

 Monatsschr. pralct. Derm., xlix, 1909, p. 95 ; LAPFONT, Bibl. Anat., 1909, 

 p. 209. 



For Trichohyatin, see GAVAZZENI, Monatsschr. pralct. Derw.,xlvii, 1908, 

 p. 229. 



658. Eleidin. To demonstrate the stratum gramilosum and 

 the eleidin granules RANVIER (Arch. Anat. Micr., iii, 1899, 

 p. 1) hardens with alcohol, stains with picro-carmine, and 

 treats with lime-water. The cells swell and show up the 

 granules, which do not change. See loc. cit., other methods 

 for the study of skin. 



Buzzi (see Encycl. mik. Technik., article " Haut ") stains 

 sections for a few minutes in a watchglassful of water with 

 2 to 3 drops of 1 per cent. Congo red. Similarly WEIDEN- 

 RKICH, Arch. mik. Anat., Ivii, 1901, p. 583. Other authors 

 recommend nigrosin, or Wasserblau, or orcein. 



See also JOSEPH, " Dermatohist. Technik," Berlin, 1905, 

 and DREUW, Med. Ktinik, Berlin, 1907, Nos. 27 and 28. 



For Cholesterin see GOLODETZ and UNNA, Monatsschr. prakf. 

 Derm., xlvii, 1908, p. 1. 



659. Horn, Hair, and Nails The elements of hairs and nails 

 may be isolated by prolonged maceration in 40 per cent, 

 potash solution, or by heating with concentrated sulphuric 

 acid. See. also VON NATHUSIUS, Zool. Anz., xv, 1892, p. 395. 



