TEGTIMKNTAUY ORGANS. 335 



at the moment of using 10 c.c. of 1 per cent, solution of 

 oosin in alcohol of 80 per cent, and 8 c.c. of 1 per cent, 

 solution of hydroqninon. Stain for five to ten minutes, 

 rinse, stain for ten minutes in 1 per cent, aqueous solution 

 of safranin, wash, treat for thirty minutes with 0'5 per cent, 

 solution of bichromate of potash, dehydrate and mount. 



Similarly DOGIEL, Folia Neurobiol. iv, 1910, p. 218 (also 

 employing Bielschowsky's neurofibril method). 



663. Corpuscles of Meissner and of Krause (Cornea and Con- 

 junctiva). DOGIEL (Arch. f. mik. Anat., xxxvii, 1891, p. 602, 

 and xliv, 1894, p. 15) employs the methylen blue method; 

 for details see previous editions. 



See also LONGWORTH'S methods, Arch. mik. Anat., 1875, 

 p. 055. 



664. Similar Objects. Papillae Foliates of the Rabbit, HERMANN, 

 see Zi-it. tr/xx. Mik., v, 1888, p. 524; ARNSTEIN, ibid., xiii, 1897, p. 240. 

 Olfactive Organs of Vertebrates, DOGIEL, Arch. mik. Anat., 1887, p. 

 74. Organs of a " Sixth Sense '' in Amphibia, MITROPHANOW, 

 Zeit. iviss. Mik., v, 1888, p. 513 (details as to staining with " Wasserblau," 

 for which see also Biol. Centralb., vii, 1887, p. 175). Nerve-endings 

 in Tongue of Prog, FAJERSTAIN, Arch, de Zool. exper. et gen., vii, 1889, 

 p. 705. Tongue of Rabbit, VON LENHOSSEK, Zeit. wiss. Mik., xi, 1894, 

 p. 377 (Ramon y Cajal's double Golgi-method). 



665. Cornea. There are three chief methods the methylen 

 blue, the silver, and the gold method. 



For the methylen blue method see particularly 345. 



Negative images of the corneal cells are easily obtained by 

 the dry silver method (KLEIN). The conjunctival epithelium 

 should be removed by brushing from a living cornea, and the 

 corneal surface well rubbed with a piece of lunar caustic. 

 After half an hour the cornea may be detached and examined 

 in distilled water. 



In order to obtain positive images of the fixed cells the 

 simplest plan (RANVIKR) is to macerate a cornea that has 

 been prepared as above for two or three days in distilled 

 water. There takes place a secondary impregnation. 



The same result may be obtained by cauterising the cornea 

 of a^living animal as above, but allowing it to remain on the 

 living animal for two or three days before dissecting it out, 



