CHAPTER XXVIII. 



l'] AND Tl'INDON (NERVE-ENDINGS). 



Striated Muscle. 



667. Muscle-cells. For these and allied subjects see, inter 

 alia, BEHRKNS, KOSSEL, und SCHIEFEERDECKER, Da* 1 Mikroskop, 

 etc., vol. ii, pp. 154 161 ; and SCHAFER, Proc. Roy. Soc., 

 xlix, 1891, p. 280. 



Iron haematoxylin gives very fine images of striped muscle, 

 and so does Mallory's phospho-tungstic. 



For dissociation methods see 527, 536, 537, 544. 



To isolate the sarcolemma SOLGER (Zeit. wiss. Mik , vi, 1889, 

 p. 189) teases fresh muscle in saturated solution of ammonium 

 carbonate. 



668. Nerve-endings the Methylen Blue Method. For BIEDER- 

 MANN'S procedure for the muscles of Astacus see 342 (see 

 also Zeit. wit>s. Mik., vi, 1889, p. 65). After impregnating as 

 there directed the carapace should be opened, and the muscles 

 exposed to the air in a roomy moist chamber for from two to 

 six hours. 



For Hydrophilus piceus, BIEDERMANN proceeded by inject- 

 ing 0'5 c.c. of methylen blue solution between the ultimate 

 and penultimate abdominal rings, in the ventral furrow, and 

 keeping the animals alive in water for three to four hours, 

 then opened the thorax by two lateral incisions, and removed 

 the muscles of the first pair of legs and exposed them to the 

 air for three or four hours in a moist chamber, and finally 

 examined in salt solution. 



G-ERLACH (Sitzb. Akad. Wiss. Miinchen, 1889, ii, p. 125) 

 injected frogs, either through the abdominal vein or through 

 the aorta, with 4 to 5 c.c. of a 1 : 400 solution in 1 per cent, 



22 



