340 CHAPTER XXVIII. 



fixed with osmic acid of 2 per cent, and impregnates it with 

 gold by the method of NABIAS, and mounts in glycerin. 



Raja. IWANZOIT (Bull. Soc. Nat. Nincou, \\, 1895,, p. 74) 

 fixes the organ in the tail of lioja with liquid of Flemining, 

 stains with hajinacalcium and eosin, and makes paraffin 

 sections. 



BALLOWIT/ (Anat. Hefte, 1 Abth., vii, 1897, p. 285) finds 

 the method of Golgi excellent for this organ. He also makes 

 sections after fixing in saturated solution of sublimate (in 

 sea-water), or in liquid of Flemming, and examines them in 

 water. Methylen-blue may be used, intra vitam. Gold is 

 little good. 



Gymnotus. BALLOWITZ (Encycl. mik. Technik., p. 303) fixes 

 with Flemming, and makes sections. He also commends 

 impregnation with gold chloride, but not the Golgi method. 



Malapterurus. BALLOWITZ (Hid., p. 202) fixes with picro- 

 sublimate, with Flemming, or with various mixtures of 

 bichromate, sublimate, and formol, and uses gold chloride 

 and Golgi impregnations. He macerates in liquid of Muller 

 or saturated aqueous solution of picric acid. 



Tendon. 



674. Tendons. RETTEEEK (C. B. 8oc. BioL, x, 1898, p. 580) 

 fixes in equal parts of saturated solutions of sublimate and 

 picric acid, puts for 1 to 3 days into saturated picric acid 

 with 2 to 3 per cent, of sodium chloride, to remove the 

 mucin, and imbeds in paraffin. 



674a. Union of Muscle and Tendon. For this see RETTEKEK 

 and LELIEVRE, C. R. Soc. Biol., 1911, No. 12 (orcein for 

 24 hours, followed by iron haematoxylin) ; and SCHULTZE 

 (Verh. phys. med. Ges. Wurzburg, 1911, p. 33) (treats for a 

 day or two with a mixture of equal parts of 2 per cent, 

 bichromate of potash and alcohol, in the dark, then for 

 2 days with 0*5 per cent, solution of hasmatoxylin in alcohol 

 of 70 per cent., then with Van Gieson's picro-saurefuchsin). 



675. Corpuscles of Golgi (RANVIKK, Traite, p. 929). Take 

 the tendon of the anterior and superior insertion of the 

 gemini muscles of the rabbit. Treat it by the formic acid 



