MUSOLF3 AND TENDON (NERVE- RNDTNns). 341 



and gold method ( 365), and after reduction scrape with a 

 scalpel, in order to remove the muscle-fibres that mask the 

 musculo-tendinous organs. 



MARCHES methods for the tendons of the motores bulbi 

 oculi (Archivio per le Scienze Mediche, vol. v, No. 15). The 

 enucleated eyes, together with their muscles, were put for 

 not less than three days into 2 per cent, bichromate of 

 potash. The muscles and tendons were then carefully 

 dissected out, stained with gold chloride and osmic acid 

 (GoLGi's method), and by the method of MANPREDI, 368. 

 Mount in glycerin. The methods only succeed completely 

 during fine, sunny weather. 



RUFFINT (Atti R. Ace. Lincei Roma Rend. [5], i, 1892, 

 p. 442) recommends the method of Fischer. 



CIACCIO (Mem. R. Ace. Sci. Bologna [4], t. x, 1890, p. 301) 

 puts tendons of Amphibia into O'l per cent, hydrochloric 

 acid or 0'2 per cent, acetic acid until transparent ; then for 

 five minutes into a mixture of 0*1 per cent, gold chloride 

 and O'l per cent, potassium chloride; then back into the 

 acetic acid, for a day in the dark, and for two or three 

 hours more in the sunlight. When they have become 

 somewhat violet they are put for a day into O'l per cent, 

 osmic acid, and finally mounted in glycerin acidulated with 

 0'5 per cent, of acetic or formic acid. 



DOQIEL (Arch. mik. Anat., Ixvii, 1906, p 638) stretches 

 tendons of eye-muscles on cardboard with hedgehog spines, 

 puts for 4 or 5 days into nitrate of silver of 1 to 2 per cent., 

 reduces for a day in pyrogallic acid with formol, and imbeds 

 in celloidin. 



Smooth Muscle. 



676. Tests for Smooth Muscle. Picro-saurefuchsin, 299, 

 stains muscle yellow, connective tissue red. 



Picro-nigrosin, 325, stains muscle yellowish, connective 

 tissue blue. 



UNNA (Encycl. mik. Technik., article " Kollagen ") stains 

 for twenty-four hours in orcein 1 part, Wasserblau 0'25, 

 alcohol 60, glycerin 10, water 30, which gives muscle in a 

 mixed tone, collagen blue, elastin reddish. See also a com- 

 plicated process with methylen blue in Monatssch. prakt. 



