348 fJHAPTFirc xxix. 



697. WEIGEIIT'S Resorcin-Fuchsin Method (Oentralb. allg. 

 Path., ix, 1898, p. 290).! per cent, of basic fuchsin and 2 

 per cent, of resorcin (or of carbolic acid) are dissolved in 

 water. 200 c.c. of the solution are raised to boiling-point 

 in a capsule, and 25 c.c. of Liquor ferri sesquichlorati P. G. 

 are added, and the whole is boiled, with stirring, for two to 

 five minutes more. A precipitate is formed. After cooling 

 the liquid is filtered, and the precipitate which remains on 

 the filter is brought back into the capsule, and there boiled 

 with 200 c.c. of 94 per cent, alcohol. Allow to cool, filter, 

 make up the filtrate to 200 c.c. with alcohol, and add 4 c.c. 

 of hydrochloric acid. 



WOLFRUM (Zeit. wiss. Mile., xxv, 1908, p. 219) adds 10 to 

 15 per cent, of acetone to the mixture. 



Stain sections (of material fixed in any way) for twenty 

 minutes to an hour, wash with alcohol, clear with xylol (not 

 with an essence). Elastic fibres dark blue on a light ground. 



MINERVJNI (Zeit. wiss. Mik., xviii, 1901, p. 161) gives a variant with 

 safranin instead of fuchsin. 



See also PRANTER, ibid., xix, 1903, p. 361; B. FISCHER, Virclnm-'a 

 Arch., clxx, 1902, p. 285, or Zeit. wiss. Mik., xx, 1903, p. 40 (chemistry 

 of the dyes obtained by these processes, which lie calls "Fudisdiu." 

 " Safranelin," etc.) ; HART, Centralb. allg. Path., xix, 1908, p. 1 ; and 

 CILIMBARIS, Arch. mik. Anat., Ixxv, 1910, p. 708. 



698. Hromatoxylin Methods. HARRIS (Zeit. wiss. Mile., xviii, 1902, 

 p. 290) makes an " Elasthaematein " as follows : Haomatoxylin 0'2 grins.. 

 aluminium chloride O'l gnns., alcohol of 50 per cent. 100 c.c., boil ;md 

 add mercuric oxide 0'6 gnns., filter and add 1 drop of HC1. Keep for 

 some weeks. Stain for five or ten minutes, put into alcohol with 1 per 

 cent, of nitric acid for one minute, then pure alcohol. 



See also DB WITT, Anat. Rec., i, 1897, p. 74 ; DUERCK, Arch. Path. 

 Anat., clxxxix, 1907, p. 62; VERHOEFF, Journ. Amer. Med. Assoc., 1908, 

 No. 11. 



MALLORY'S phosphotungstic hsematoxylin is good, but not specific. 



699. Other Methods for Elastic Tissue : 



For the elastic tissue of the skin see PASSARGE and KROSINO, T)n-m. 

 Stud., xviii, 1894. 



See also for staining and dissociation AGABABOW, Arch. mik. Anal., 1, 

 1897, p. 566 etseq. 



For C. MARTINOTTI'S silver impregnation Bee Zeit. wiss. Mik., v, 1888, 

 p. 521, or Arch. Hal. Biol, xi, 1889, p. i>:>7. 



SCHUMACHER (Arch. mik. Anat., lv, 1899, p. 151) Ims ].;ul good results 

 (for the spleen) with piVro-nigroHin. <JSl. 



See also 7: 1: 1. 



