362 CHAPTER 



a few seconds drops them face downwards into a solution 

 of 



Absolute alcohol saturated with eosin . 25 c.c. 

 Pure ether . . . . 25 



Sublimate in absolute alcohol (2 gums. 



to 10 c.c.) ..... 5 drops. 



After three or four minutes they are washed, stained, and 

 mounted in balsam. 



For JENNEK'S fixing and staining and staining method, see 

 next . 



Many recent authors fix wet films with formal. BENAKIO 

 (Deut. med. Wochensclir., 1895, p. 572) mixes 1 part of 10 per 

 cent, formol with 9 of alcohol (the mixture must be freshly 

 prepared), and plunges films into it for a minute. 



Similarly GULLAND, with 1 part of formol to 9 of alcohol. 



Similarly WEKMEL (see Zeit. wixs. Mik. t xvi, 1899, p. 50), 

 who combines various stains (methylen blue, eosin, gentian, 

 etc.) with the formol. 



EDINGTON (Brit. Med. Journ., 1900, p. 19) exposes films 

 for 15 to 30 minutes to vapour of formol under a bell-jar. 



SCOTT (Juurn. of Path, and Uacter., vii, 1900, p. 131). 

 exposes films to the vapour for about -5 seconds mid drops 

 into absolute alcohol, and after 15 minutes stains and mounts. 



A short exposure (30 seconds) to vapour of osmium has 

 also been recommended. 



720. Stains for Blood. Fredi (unfixed) blood can be stained 

 on the slide. 



TOISON (Jovrn. Sci. nu'd. de Lill?, fev., 1885 ; Zeit. y //**. 

 Mile., 1885, p. 398) recommends that it be mixed with tin- 

 following fluid : 



Distilled water . . .160 c.c. 



Glycerin (neutral, 30 Baunirj . 30 



Pure sulphate of sodium . . 8 grammes. 



Pure chloride of sodium . . 1 gramme. 



Methyl violet 5 B . . 0'25 



(The methyl violet is to be dissolved in the glycerin with 

 mir half of the water added to it; the two salts are to be 

 dissolved in the other half of the water, and the two solu- 

 tions are to be mixed and filtered.) This mixture stains 

 leucocytes sharply, which facilitates enumeration. 



