BLOOD AND C4LANDS. 363 



BIZZOZKKO and Ton RE (Arch. Sci. Mediche, 1880, p. 390) 

 dilute a drop with normal salt solution containing a little 

 methyl violet, which stains nuclei intensely, cytoplasm less 

 intensely. 



Similarly GiGLio-Tos (Zeit. ivixs. Mik., 1898, p. 166), 

 diluting with saturated solution of neutral red in salt 

 solution, which stains ha3inoglobigenous granules in five to 

 ten minutes. This is also recommended by EHRLICH and 

 LAZARUS, see 309. 



Similarly also Ross (Trans. Path. Soc., 1907, p. 117), 

 using polychrome methylen blue. 



LBVADITI (Journ. Phys. path. Gen., Paris, 1901, p. 425) 

 allows solution of Brillantkresylllau in alcohol to dry on 

 a slide, puts a drop of blood on the dried layer, and 

 covers. Similarly CESAKIS-DEMEL (Arch. path. Anat., 1909, 

 p. 92), with a mixture of this dye and Sudan III ; and 

 NAKANISHI (Centralb. Bakt., 1901, p. 98), with methylen 

 blue BB. 



Fixed films may be treated with the usual tissue stains, 

 rosin being an important one, as it stains rose-red all parts 

 of blood-cells that contain haemoglobin. EHKLICH'S acid 

 tematoxylin, with 0*5 gr. of eosin dissolved in it, is a good 

 general stain. Or, stain with hoernalum, and then with eosin 

 (0*5 per cent, in alcohol or water). 



EURLICH'S triacid, 296, gives good general views, and 

 demonstrates neutrophilous granules. His mixture for eosino- 

 philous cells has been given, 311. 



PAPPENHEIM'S panoptic triacid (on sale by Grubler) is 

 Ehrlich's triacid with methylen blue in place of the methyl 

 green. 



CHENZINSKI'S mixture, which is good, has been given, 313. 

 Stain for six to twenty-four hours in a stove. This gives 

 rise to precipitates. To avoid them (WILLEBKAND, Deutsch. 

 med. Wochenschr., 1901, p. 57) you may make a mixture of 

 equal parts of 0*5 per cent, solution of eosin in 70 per cent, 

 alcohol and saturated solution of methylen blue in water, 

 and add acetic acid of 1 per cent, drop by drop till the 

 mixture begins to turn red, and filter before use. Or 

 (MICHAELIS, Hid., 1899, No. 30) make (a) a mixture of 20 parts 

 1 per cent, aqueous methylen blue with 20 of absolute alcohol, 

 and (6) a mixture of 12 parts 1 per cent, aqueous eosin with 



