366 CHAPTER XXX. 



See also, for paraffin sections, SCHUBERG, in Deutuch. med. 

 Wochenschr., xxxv, 1909, No. 48, or Zeit. wiss. Mile., xxvii, 

 1910, p. 161, who passes through acetone and xylol into 

 balsam. 



The older Romanowsky stains published by the authors 

 mentioned above, as also Laveran's f{ Bleu Borrel " seem to 

 be superseded by Griemsa's. 



LEISHMAN'S Romanowsky Stain (Brit. med. Jo urn., March 

 16th and September 21st, 1901) is as follows : To a 1 per cent, 

 solution of Griibler's medicinal methylen blue in water add 

 0*5 per cent, of sodium carbonate, heat to 65 C. for twelve 

 hours and let stand for ten days. Then add an equal 

 volume of O'l per cent, solution of Griibler's Eosin extra B, 

 let stand for six to twelve hours, collect the resulting pre- 

 cipitate on a filter, wash it until the wash water comes off 

 colourless, dry and powder. For staining, dissolve 0'15 grtn. 

 in 100 c.c. of pure methyl alcohol. Stain cover-glass films 

 (air-dried) for five to ten minutes ; flood the film with water 

 for one minute, and examine, or dry (without heat) and 

 mount in xylol balsam. Nuclei in shades of red, cytoplasm 

 lilnish, parasites blue with ruby red chroma tin. 



RAADT (Miincli. med. Wochenschr., 1911, No. 27; Zeit. 

 wixs. Mik., 1912, p. 236) obtains a Romanowsky stain of 

 blood and parasites with JKNNER'S solution. Films fixed 

 with alcohol and ether are first stained for five to ten 

 minutes in solution of one part methylenblau med. puriss. 

 Hoechst, 0'5 part of lithium carbonate and 100 of wafer, 

 kept for at least three weeks and diluted with 10 vols. <>l 

 water. Rinse with water, dry with blotting paper, flood 

 with JKNNEIC'S solution diluted with 2 or 3 vols. of wjitrr, 

 and stain for five to ten minutes. Wash, dry with blot! inn- 

 paper, and mount. 



721. PAITENHEIM (Annt. Anz., xlii, 1912, p. 525) recommends the 

 following for sections of //.rwn/Wefe'c tissues, :m<l also of kidney, liver, 

 !ivi>..)> Lysis, supra renals, lung, Intestinal epithelium and central nervous 

 in. Fix in Orlli's Formol-Miiller, stain sections f or twenty minutes 

 in a stove in " aqueous diluted alcoholic " solution of MAY-GR.UNWALD 

 or JENNKR diluted with 8 vols. of water; after-stain for forty minutes 

 in the stove in " ;nMieoiis (IIKMSA solution (P> C.e. of water with 10 

 drops of glacial aeetie arid)"; differentiate in 100 c.c. of water with 

 :> to (> drops of acetic acid ; wash, dry between blotting-paper ; dehydrate 

 in mixture of e. | ual parts of ae<'toin andjal>solute alcohol, and mount in 



