368 CHAPTER XXX. 



then thoroughly removed by means of xylol, and a drop of 

 balsam and a cover are added. This stain may be applied 

 to celloidin sections without previous removal of the celloidin. 



See also the modifications of this method by KROMAYER ( 656) ; 

 BENECKE ( 690) ; UNNA (Monatssch. prakt. Dennat., xx, 1895, p. 140) ; 

 WOLFF (Zeit. wiss. Mile., xv, 1899, p. 310) ; and one of another sort by 

 KOCKEL, Centralb. allg. Path., x, 1899). 



Glands. 



724. Mucin. HOYER (Arch. mik. Anat., xxxvi, 1890, p. 310) 

 finds that the mucin of mucus cells and goblet cells stains 

 with basic tar colours and with alum ligematoxylin, but not 

 with acid tar colours. He obtained his best results by means 

 of thionin, and good ones with toluidin blue, both of these 

 giving a metachromatic stain tissues blue, mucin reddish 

 and also with methylen blue (which is particularly useful 

 from its power of bringing out the merest traces of mucin), 

 safranin, etc. 



Tissues should be fixed for two to eight hours in 5 per 

 cent, sublimate solution, and paraffin sections stained for 

 five to fifteen minutes in a very dilute aqueous solution of the 

 dye (two drops of saturated solution to 5 c.c. of water). 



Hyaline cartilage, the jelly of Wluirton, and the Mastzellen 

 of Bhrlich give the same reactions with basic dyes as mucin 

 does. 



See also SUSSDORF, Deutxche. Zeit. Thiermed., xiv, pp. 345, 

 349 (Zeit. wis*. Nik., vi, 1889, p. 205) ; BI//O/KKM, Alii. If. 

 Accad. di Sci. di Torino, 1889 to 1892 (reports in Zeit. wiss. 

 Mfc., vii, 1890, p. 61 ; and ix, 1892, p. 219) ; also UNNA, v'/m/., 

 xiii, 1896, p. 42. 



The safranin reaction is not obtained with all brands of 

 the dye; that of Bindschedler and Uusch, in Bale, gives it, 

 whilst safranin Oof Griibler dues not. UNNA employs cliiclly 

 polychrome methylen blue. 



As rrgjirds tlie lliioniji stnin, sec HAKI, Arch. Mil:. Aimt., 



Iviii, 1901, p. 678. 



BRUNO (Hull. Nor. Nat. NapoU, 11)05, ]>. 220) fixes and 

 stains tlie skin of the frog in :i mix! lire of 1 00 c.c. of formol 

 of 1'25 ])(! crnt. with S c.c. of I percent, solution of thioniii. 

 Mucus glands red. 



