372 CHAPTER XXX. 



See also for the thy runs some very complicated methods of 

 SALKIND, Anat. An?,., xli, 1912, Nos. 6 and 7. 



735. Kidney . SAUEK (Arch. mik. Aiiat., xlvi, 1895, p. 110) 

 finds that for the renal epithelium the best fixative is Carney's 

 acetic alcohol with chloroform (three to five hours, washing 

 out with absolute alcohol). A mixture of nine parts alcohol 

 with one of nitric acid is also good, as is liquid of Perenyi. 

 He stains with iron haematoxylin, and after-stains in a very 

 weak solution of Saurerubin in 90 per cent, alcohol, which 

 stains the ciliary plateau. He macerates with iodised 

 serum or one-third alcohol, staining afterwards with dahlia. 



ARNOLD (Anal. Anz., xxi, 1902, p. 417) employs intra vitam 

 staining methods for the study of the granules of the 

 epithelium cells. Sections of fresh kidney are cut with a 

 Valentin's knife, and brought into a very dilute solution of 

 neutral red, or methylen blue, in which the granules stain in 

 a few minutes or hours. Or saturated solutions of the dyes, 

 or of indigo carmine, may be injected subcutaneously during 

 life, at intervals of fifteen to twenty minutes, and after two 

 to five injections the organ may be excised and sections 

 made and examined (see 208 and 342 to 344). 



