NEltVOUS S VS'I 1 KM UKXKK'AL METHODS. 385 



>t' w;itvr. and iOO of alcohol, washes with alcohol, clears with origanum 

 oil, and mounts. 



751. Alizarin. SCHKOTTER (Neurol. Centralb. } xxi, 1902, 

 p. 338 ; Zeit. iciss. Mik., xix, 1903, p. 381) stains sections 

 for twenty-four hours in a 1 to 2 per cent, solution of sul- 

 phalizarinate of soda, differentiates for -- to 1 minute in 

 tap-water, dehydrates, and mounts. This is a general stain, 

 but demonstrates Nissl bodies and other internal details. 



752. NISSL'S methylen-blue is used as a general stain by some. 



ROTHIG (Folia NeurobioL, ii, 1909, p. 385 ; Zeit. wiss. Mile., xxvi, 1909, 

 p. 2S2) fixes and stains for about four weeks in saturated solution of 

 Mctlujlenazur I (Griibler) in formol of 10 per cent., puts for ten to fifteen 

 minutes into aceton, then for twelve hours into chloroform, and imbeds 

 in paraffin. He also has a process with trichloracetate of lead and 

 methylenaztir. 



RAWITZ (Zeit. wiss. Milt., xxvi, 1910, p. 341) has some complicated 

 methods with Indidin, Indamin blue, and Azosdnreblau, which take 

 twenty-eight days ; and (ibid., xxviii, 1911, p. 1) others wiihfuchsin and 

 azofuclisin which take over thirty-six days. 



KAPPERS (ibid., p. 417) describes a stain of chromic material with 

 extract of elderberries. 



SCARPATETTI (Neurol. Centralb., xvi, 1897, p. 211 ; Zeit. wiss. Mik., 

 xiv, 1897, p. 91) stains sections of formol material for five minutes in 

 1 per cent, hsematoxylin, treats for five minutes with concentrated 

 solution of neutral acetate of copper, differentiates with Weigert's 

 borax-ferri-cyaiiide, treats with concentrated solution of carbonate of 

 lithia, washes and mounts. Myelin is not stained. 



753. M ALLORY'S Phospho-molybdic-acid Haematoxylin has been 

 given, 271. 



For the extremely complicated modification of AUERBACH, see Neurol. 

 Centralb., xvi, 1897, p. 439, or Zeit. wiss. Mik., xiv, 1897, p. 402, and for 

 that of KODIS see 271. 



754. Haematoxylin and S'iurefuchsin. FINOTTI (Virchow's Arch., 

 cxliii, 1896, p. 133 ; Zeit. wiss. Mik., xiii, 1896, p. 236) stains in hamia- 

 toxylin, counter- stains for three minutes with 0'5 to 1 percent, solution 

 of Siiurefuchsin, and differentiates in 75 per cent, alcohol containing a 

 very little caustic potash. 



VAN GIBSON'S haematoxylhr and picro-Saurefuchsin, 398, gives 

 useful general views of nerve-cells, axis-cylinders, and neuroglia. 



755. ALT (Miincli. med. Wochenschr., 1892, No. 4; Zeit. wiss. Mik., 

 ix, 1, 1892, p. 81) stains for a couple of hours in solution of Congo red 

 in absolute alcohol, and washes out with pure alcohol. For peripheral 

 axis -cylinders, and other elements. 



25 



