392 CHAPTER XXXII. 



They remain in this for twenty-four hours. They are then 

 washed, hardened in alcohol, imbedded in celloidin or paraffin, 

 and sections mounted in damar. 



TELLYESNICKY (Verh. Anat. Ges., 1904, p. 183) advises toning the 

 sections for five to thirty minutes in 150 c.c. of water with 4 c.c. of 

 1 per cent, gold chloride. This is good for weak impregnations, Imt 

 not desirable for strong ones which show good contrast. 



Sections from the outer layers are too dark for study, 

 those from the innermost too pale (if the specimens arc 

 large ones), whilst those from intermediate layers are fit for 

 study. The over-stain of the outer layers can be diminished 

 by diluting the silver bath with 1 volume of water for the 

 last twelve hours. 



This method has the defect of giving an imperfect fixation 

 and impregnating almost exclusively cell-bodies and dendrites. 

 It is not good for the large cells of adults, but excellent for 

 small and medium cells of newborn or very young subjects, 

 and for very early embryos in general. 



Formula la A. As last, but with nitrate of silver of 3 to 

 6 per cent. 



DOGIEL (Anat. Anz., xxv, 1904, p. 558) finds this method 

 gives results not attainable by other means in the study of 

 the corpuscles of GRANDRY (stoving for four to six dnys). 

 Similarly KOLMEK (ibid., xxvi, 1905, p. 560) with epiderm of 

 Lumbricus, etc. ; and other authors for the ganglionic cord 

 of Hirudinea. 



Formula la B.^ As before, but nitrate bath of only 0*75 

 per cent., and very small pieces of tissue, preferably embryos 

 and newborn subjects. Poor fixation, much shrinkage, but 

 vigorous stain of neurofibrils, of nucleolar granules, and of 

 the intra-nuclear rodlet of KONCORONI. 



Formula la C. As before, but silvering in nitrate of 2 per 

 cent, with one fourth of absolute alcohol or acetone added. 

 Better fixation than pure nitrate. Gives results very similar 

 to those of la with dog, cat and rabbit, and better results 

 with human cerebrum and cerebellum. 



Formula 2a. Fixation for 24 hours in alcohol of 96 per 

 cent. Tissues not washed, but mopped with blotting- 

 paper, and put into nitrate of silver of 1*5 per cent, for 

 seven days at 35 C., <>r six days at 40 C. The rest. MS 



