CHAPTER XXXIII. 



MYELIN STAINS. 



779. Iron Haematoxylin. I find the simplest way to make 

 n myelin stain is to make paraffin sections of formol material 

 and stain them with iron hasmatoxylin, exactly as for central 

 corpuscles (say twelve to fourteen hours in the mordant, six 

 in the hasmatoxylin, and about two minutes for the differen- 

 tiation). Sections best not over 15 /n. You may after-stain 

 the cells (which are only grey) with carmalum, but not for 

 more than half an hour, or the hgematoxylin will be attacked. 

 The stain is not so resthetic as Weigert's, but quite as sharp. 

 Axis cylinders are not shown. 



Similarly REGAUD, C. R. Acad. Sci., clxviii, 1909, p. 861, 

 but adding a chrome inordantage either concurrently with the 

 formol fixation, or subsequently. 



Also NAGEOTTE, C. R. Soc. Biol , Ixvii, 1909, p. 542, with 

 sections of formol material by Unfreezing method; HOUSE R, 

 Jo urn. Comp. Neural, and Psych., x, 1901, p. 65, and 

 BROOKOVEU, ibid., xx, 1910, No. 2 ; SPIELMEYEE, Neurol. 

 Zentralb., xxix, 1910, p. 348, and his Technik. d. mikro. 

 Untersuch. d. Nerventystems, 1911, p. 87, with sections of 

 25 to 85 ji by the freezing method ; LOYEZ, C. R. Sac., Biol., 

 Ixix, 1910, p. 511, who differentiates first lightly, till the 

 grey begins to come out, in the iron alum, then washes, 

 and differentiates further in Weigert's borax ferricyanide ; 

 GILBERT, Zeit. u-iss. Mik., xxviii, 1911, p. 279, who mordants 

 with iron alum, stains with molybdic acid hsematoxylin, and 

 differentiates with the borax ferricyanide ; STOELZNER, ibid., 

 xxiii, 1906, p. 329, who mordants celloidin sections of formol 

 material for five minutes in Liq. ferri sesquichlorati, stains 

 in haematoxylin of 0'5 per cent., and differentiates in the 

 mordant or in borax ferricyanide; and KODIS, Arcli. mik. 

 Auat., lix, 1901, p. 211, who fixes for one or two days in 



