AXlS-CVLINDEli AND DENDIilTE STAINS. 



belluin. It is superior to the silver method in that the 

 reaction can always be obtained with perfect certainty in a 

 certain time ; that the preparations can be perfectly pre- 

 served by the usual methods ; and that large pieces of tissue 

 can be impregnated. It generally gives a more abundant 

 impregnation than the silver method. 



See also FLATAU, in Arch. mik. Anat. } xlv, 1895, p. 158. 



Modifications of Golyi's Bichromate and Sublimate Method. 



828. PAL [Erratim, " Tal," loc. cit.~] (Gazz. degli Ospitali, 1886, No. 68) 

 finds that if sections made by this process be treated with solution of 

 sodium sulphide, a much darker stain is obtained. Sections may then 

 advantageously be double-stained with Magdala red. 



Golgi's method may be combined with Weigert's nerve stain (see PAL, 

 Wien. med. Jahrb., 1886 ; Zeit. wiss. Mik., v, 1887, p. 93). 



For FLECHSIG'S modifications, see Arch. Anat. Phys., Phystol. Abtli., 

 1889, p. 537. 



829. Cox (Arch. mik. Anat., xxxvii, 1891, p. 16) finds the 

 sublimate and bichromate may be used together. He used a 

 fluid consisting of 20 parts 5 per cent, bichromate, 20 parts 

 5 per cent, sublimate, 16 parts 5 per cent, simple chromate 

 of potash, and 30 to 40 parts of water. (The chromate 

 should be diluted with the water before adding it.) The 

 mixture should be as little acid as possible. The pieces of 

 tissue should be small. The duration of the impregnation is 

 from two to three months. There is considerable difficulty 

 in preserving sections, which are best made with a freezing 

 microtome, alcohol being avoided, treated for an hour or two 

 with 5 per cent, solution of sodium carbonate, and mounted 

 icithoiit a cover j in a medium composed of gum sandarac, 

 75 gr. ; camphor, 15 ; oil of turpentine, 30 ; oil of lavender, 

 22'5 ; alcohol, 75; castor oil, 5 10 drops. For examination, 

 add a drop of castor oil, and cover. 



Dr. A. SANDERS writes me (June, 1898) that the stain keeps very well 

 if the sublimate be well removed by washing in many changes of alcohol, 

 and the tissues passed through alcohol and ether into celloidiii, and the 

 sections mounted in chloroform -balsam under a cover. I find the stain, 

 keeps ; but the preparations quickly develop opaque granules that are 

 very undesirable. 



BREMER (Anat. Rec., 1910, p. 265) cuts in celloidin and stains with 

 alum haematein and eosin. 



28 



