436 CHAPTER XXXIV. 



centrated methylen blue into the body-cavity of FISHES, 

 removes the brain after half an hour,, puts slices of it into 

 the same solution for half an hour, and fixes as usual. 



LENNHOFF (Neurol. Zentrall., 1910, p. 1) has some com- 

 plicated methods with polychrome metliylen blue and 

 sulpho-cyanide of potassium, or ferricyanide. 



834. LENNHOFF'S Iron Method (ibid.). Sections put for 

 thirty seconds into 2 c.c. of 15 per cent, solution of tannin 

 with 3 drops of 5 per cent, solution of oxalic acid, rinsed in 

 water, then for a few seconds in 1 per cent, solution of 

 chloride of iron till no further blackening occurs, then 

 washed, dehydrated and mounted in balsam. Axis cylinders 

 black, cells grey. 



835. FAJERSTAJN'S Haematoxylin (Pohi. Arch. Biol. Med. 

 Wiss., i, 1901, p. 189). Sections by the freezing method of 

 material fixed for two to seven days in formol of 5 to 10 

 per cent, mordanted for five to twenty-four hours in chromic 

 acid of 0*25 to 0'5 per cent., well washed, stained for twenty- 

 four hours in 1 per cent, aqueous ha3matoxylin, and differen- 

 tiated by the method of PAL. 



Other Methods. 



836. NABIAS (C. R. Soc. Biol., Ivi, 1904, p. 426) treats 

 sections until yellow with solution of 1 grin, iodine and 

 2 grins, iodide of potassium in 300 of water, washes, treats for 

 a few minutes with 1 per cent, chloride of gold, washes and 

 reduces in anilin or resorcin in water (1 : 100, or less for the 

 latter) and mounts in balsam. 



APATHY'S Gold Method lias been given 371. 



GKRLACH'S Bichromate and Gold Process lias been given. 369. 



For ;i c< implicated Gold Method of RAMON Y CAJAL, see Rev. trim. 

 Micr., v, 1900, p. 95 ; or Zrit. u-i. Mile., xix, 1902, p. 187. 



For UPSON'S exceedingly complicated Gold and Iron and Vana- 

 dium Methods see MKHCIKR, in Zeit. wiss. Mik., vii, 1891, p. 474; or in 

 liis Coupes da Syntmie Nerveux Central, p. 2o4; or early edition*. 



For FAJEKSTAJN'S complicated Silver Method see Neural. Centralb., 

 xx, 1901, p. 98 ; or Zeit. wiss. Mik., xviii, 1901, p. 214. 



MAGINI'S Zinc Chloride Process (see Soil. Accad. Mcd. di Roma, 

 1886 ; Zeit. wiss. Mik., 1888, p. 87, or early editions). 



