NEUROGLIA, AND SENSE ORGANS. 445 



GOLGI'S sublimate impregnation (Cox's form) has also been 

 successfully employed by KKAUSK and RAMON. 



The bichromate-and-silver method serves for the study of 

 the fibres of Miiller and neuroglia cells, as well as neurones. 

 Weigert's neuroglia stain does not give good results. 



LENNOX (Arch.f. Opthahn., xxxii, 1 ; Zeit. wiss. Mik., iii, 1886, p. 408) 

 has used Weigert's ha3matoxjliii method. 



KUHNT (Jen. Zeit. Naturw., Bd. xxiv, H. 1, 1889, p. 177) employs 

 Pal's modification. Similarly SCHAFFER (Sitzb. Alcad. wiss. Wien., xcix, 

 1890), 3, p. 110 ; Zeit. wiss. Mik., viii, 1891, p. 227). These methods give 

 a differential stain of rods and cones. 



For the zoiiula and ciliary body see MAWAS, Arch. d'Anat. micr., xii, 

 1910, p. 103. 



847. Dissociation. For maceration preparations you may 

 use weak solutions (0'2 to 0*5 per cent.) of osmic acid for 

 fixation, and then macerate in 02 per cent, chromic acid 

 (M. SCHULTZE), or in iodised serum (M. SCHULTZE), or in 

 dilute alcohol (LANDOLT), or in Miiller's solution, or (RANVIER, 

 Traite, p. 957) in pure water, for two or three days. THIN 

 (Journ. of Anat. y 1879, p. 139) obtained very good results 

 by fixing for thirty-six to forty-eight hours in one-third 

 alcohol, or in 25 per cent, alcohol, and then staining and 

 teasing. 



SCHIEFFERDECKER macerates fresh retina for several days in 

 the methyl mixture, 543. 



KRAUSE (Intern. Monatsschr. Anat., 1884, p. 225) recom- 

 mends treatment for several days with 10 per cent, chloral 

 hydrate solution ; the rods and cones are well preserved. 



Inner Ear. 



848. Inner Ear, Dissection. For the dissection of the human ear 

 see POUTZER, " Die auatomische u. histologische Zergliederung d. men- 

 schlichen Gehorogaiies," Stuttgart (Enke), 1889 (Zeit. iviss. Mile., vii, 

 1890, p. 364). Amongst the lower mammalia, the yuinea-piij is a 

 favourable subject, as here (as with some other rodents) the cochlea 

 projects freely into the cavity of the bulla, and may be easily removed 

 with a scalpel and brought into a fixing liquid, and opened therein. 

 With fishes and amphibia also the membranous labyrinth may easily 

 be got away. 



849. Preparation. SCUWALBE (Beitr. z. Phyts., 1887; Zeit. 

 wiss. Mik., iv, 1887, p. 90). Fix (cochlea of guinea-pig) for 



