NKUUOULIA, AND SENSE ORGANS. 447 



of 0'5 per cent, to 1 per cent, solution every five minutes 

 through the vena femoralis, until the death of the animal. 

 The cochlea then to be got out, exposed to the air for 15 or 

 30 minutes, and fixed for some hours (overnight) in 10 per- 

 cent, ammonium molybdate with a little osmic acid. Then 

 decalcified in trichloracetic acid of 5 per cent, with a trace 

 of platinum chloride, washing for 24 hours and got into 

 paraffin. 



For fishes and amphibia the immersion method will suffice. 



850. Other Methods. WALDEYER, Strieker's Handb., p. 958 (de- 

 calcification either in O'OOl per cent, palladium chloride containing 10 

 per cent, of HC1, or in chromic acid of 0'25 to 1 per cent.). 



URBAN PRITCHARD (Journ. Roy. Mic. Soc., 1876, p. 211). Decalcifi- 

 cation in 1 per cent, nitric acid. 



LAVDOWSKY (Arch. mik. Anat., 1876, p. 497). Fresh tissues (from the 

 cochlea) are treated with 1 per cent, solution of silver nitrate, then 

 washed for ten minutes in water containing a few drops of O5 or 1 per 

 cent, osmic acid solution, and mounted in glycerin. 



MAX FLESCH (Arch. mik. Anat., 1878, p. 300) ; TAFANI (Arch. Ital. de 

 Biol., vi, p. 207) ; EICHLER, Abh. math-phys. Ci. Sachs. Ges. Wiss., xviii, 



1892, p. 311 ; Zc.it. wiss. Mik., ix, 1893, p. 380 (injection of blood-vessels 

 of the labyrinth) ; SIEBENMANN, Die Slatyefdsse im Labyrinths des 

 ineiischli'.-htfii Ohres, Wiesbaden, Bergmaim, 1894 ; Zeit. wiss. Mile., xi, 

 1894, p. 386; GRAY, Joiini. Anat. Phys., 1903, p. 379; SCOTT, ibid., xliii, 

 1909, p. 329. 



851. Olfactive Nerve-endings, Tactile Corpuscles, etc. 

 Besides the gold method, Chapter XVII, and the metkylen- 

 blue method, Chapter XVI, the rapid bichroiuate-and-silver 

 method of GOLGI should be employed, and for the olf active 

 inucosa gives the best results ; see VAN GUHUCHTEN, La Cellule, 

 vi, 1890, p. 405. For intra-epidermic nerve-endings, besides 

 the methods given Chapter XXVII, the GOLGI method should 

 be employed. According to VAN GEHUCHTEN (La Cellule, ix, 



1893, p. 319) it gives much better results than gold methods. 

 He uses the rapid process. For tactile corpuscles, etc., besides 

 the methods given 661 and 662., see RAMON Y CAJAI/S neuro- 

 iibril methods. 



