\\.\\l. 

 , /'/-,/-,/ Unit'. Neapel, \.\vii, IDOJ, p. 292) 



n;i]VMti>r> Mytilid'e with '2 per cent, of cocaine in sea vvah-r, 

 ;ind (fur preservation of cilia) fixes in sea water, with 10 per 

 mil. <>f fnrmol. 



Lo BIANCO advises that I Yosobra nchiat a, and, amongst 

 the Heteropoda, Atlantida-, In; n;ircot ised with 70 per cent. 

 alcohol, 18. For Opiftthobranchiata I recommend sudden 

 killing witli liquid of IVn'nyi, or the acetic method, 

 Af>li/xta may first. l>e na.rcot is(>d l>y snhciitaneous injection of 

 about 1 c.c. of a 5 to 10 per cent, .solution of hydrochlorate 

 of cocaine (KoiiKiM 1 , Jjnlf. ,SV/V///. <!,. hi, /<'num- t etc., 1<S(M), 

 p. 449; Zeit. wisn. mik., ix, 1892, p. 216'), or (SI,'HONI,KIN, 

 /'/'/. HioL, xxx, 1893, p. 187) 1 c.c. of 4 per cent, solution of 

 I'elletierin. For Lo UIAVM'S various methods nee the 

 original, ]>. lu. 



For Pteropoda in general, li(|iiid of Perenyi. drw.tx is a 

 difficult form. Lo BIANCO advises the alcohol method, IS. 

 For the (lymnos<>mata he narcotises with 0' 1 percent, chloral 



hydrate. 



For terrestrial Gastropods see ^ and jl(j. M.\i;riii 

 (Arch. i)rik. Anat., 1867, p. 204) gets rid of the mnci. 

 the internment of Limax, which may he an ol^taci 

 preparation, by putting the living animal into moderately 

 concentrated salt solution, in which it throws off its mucus 

 and dies in a few hours. 



L\W(Anat. Hefte, 1902, p. 84) puts Helix into water with 

 enough chromic acid to make it of n Rhine-wine colour, 

 with an air-tight cover to the vessel, and when the animals 

 are extruded injects into them a quarter to a half of a 

 I'ravaz syringe of 1 per cent, cocain, and after five to fifteen 

 minutes dissects and fixes. 



Hi VMANS (Bull. Acad. Bely. t xxxii, 1S96, p. ."7n) injects 

 ethyl bromide under the skin of (,'ephalopoda. 



Lo BIANCO uses for fixing them his chromo-acetic acid, 

 No. 1 ( 39), with a double quantity of acetic acid, for 

 uty-four hour-. 



856. Liver of Mollusca. KNIM^T-KS (Mltth. Zuol. 



, xv, l'..0l, p. 289) fixes the liver of (}rt(,pns and 

 ith sublimate. For A/'li/^ia (especially in summer; 

 hoi, formol, and chromic mixtures are counter-indicated, 



