\u;rimi.s KOI: iNVKiM'i'iKUATKs. I l 



on account ol' the carbohydrate! in the cell. Sublimate is 

 best. 



857. Nervous System of Pulmonata. U. de NAIHAS (.!</. ,SV. 



Linn. Bordeaux, 1S!M- ; /Mr//. ///*/. <v////v.v urn-fur f/r.v 

 (i'nst< : roi>'nlr}i t IS'.H, p. 23) opens I lie animals ami lixes I lie 

 ganglia Tor one hour in JL mixture of (i pails glacial acetic 

 acid to 100 of 90 pur cent. alcolml, <r l'..r fifteen to twenty 

 minutes in 5 per cent, sublimate with 5 per cent, acetic acid. 

 lie stains in bulk, with Honaut's luumatoxylio oosin, or 

 U. lleidenhain's luomatoxylin, or a copper h;ematoxylin ol 

 Viallanes, ^7-I-, and imbitds in parallin. Ho also stains by 

 the rapid method ol (Jnuii, imbedding, huwovor, tho ^an^lia 

 in cdloidin directly after the hardening in osmii; ac-id and 



bichromate, and treating the sections with tho silver (p. ;M<). 

 lie stains with methylon bine by treating tho ganglia iiixiln, 

 for twelve to twenty-roiir hours with a 1 per cent, solution. 



DiiKYKii (/''//. iritis. ZuoL, xcvi, 1910, j). 380) narcotises 

 Nudibranchs with cocain, and Tor studying tho uorvus 1 

 them with MAYKK'S picro-lonnol, }>uts loi- a week into a 

 mixture of 1 gnu. of iron alum with 2 c.c. of formol and 40 

 of water, makes sections and stains with iron h;einatoxylin. 



See also, for nerve-cells, McCLUliK, Hoot. Jtikrb., 1S9H, 

 p. 17 (MANN'S methyl blue and rosin, or UKNDA'H satVanin 

 and Ijichtgriia), and LEGENDHK, Arch. mic. Anat., x, 1909, 

 p. 81l>. 



858. Eyes of Gastropoda (CLEMMING, ,-I/r//. mik, Amil.., 1870, 

 p. 441). r l'o oljtain the excision of an exserted eye, make a 

 rapid cut at tho baso of tho peduncle, and throw tho organ 

 into very dilute chromic acid, or 4 per cent, bichromal , 

 after a short time it will evaginate, and remain as completely 

 erect as if alive. Harden in 1 per cent, osmn- acid, in 

 alcohol, or iu bichromate. 



SMITH (Bull. Mus. Comp. /not.. Harvard, xlviii, 1906, 

 p. 238) macerates eyes for at least two days in 9 parts of 

 water with I of weak mi.xnro of Flemming, followed by 

 glycerin of 10 per cent. lie bleaches thorn (in sections) 

 with nitric acid and chluralo of potash. 



859. Eyes of Cephalopoda and Heteropoda ((IKKNACJIKI;, /1//A. 

 naturf. <!< . ll'dlc-u.-ti., Bd. xvi, 1890, p. 213).- JJupigm. m 



