458 CHAPTER XXXVI. 



hours (Myriopoda) into a mixture of 2 parts of 80 per cent, 

 alcohol with one of glycerin and 2 per cent, of nitric acid, 

 best kept at 35 C. The elements are well preserved. 



WIDMANN (Zeit. wiss. ZooL, xc, 1908, p. 260) makes the 

 lens of Arachnida fit for sectioning by putting for a day or 

 so into alcohol with 10 to 15 per cent, of nitric acid ; and 

 bleaches sections with 1 part of chlorine water to 2 of alcohol. 



See also ROSENSTADT, Arch. mile. Anat., xlvii, 1896, p. 478 ; 

 VIALLANES, Ann. Sci. nat.j xiii, 1892, p. 354 ; and DIETRICH, 

 Zeit. wistt. Zool., xcii, 1909, p. 465 (fixes in alcoholic formol, 

 and bleaches with dilute aqua regia). 



875. Injections (Arachnida and Crustacea especially). 

 AIME SCHNEIDER (Tablet tes ZooL, ii, 1892, p. 123) recom- 

 mends lithographic Indian ink, the animals being narcotised 

 with chloroform, then injected and thrown into strong alcohol. 

 Similary CAOSARD (Bull. 8c. France Belg., xxix, 1896, p. 16). 



876. Arctiscoida (DoYERB, Arch. mik. Anat., 1865, p. 105). EXM- 

 iniiiation of living animals after partial asphyxiation in boiled water. 

 See early editions. 



Verm es. 



877. Chaetopoda: Cleansing Intestine. KUKENTHAL (Journ. 

 Roy. Mic. 8<,c, 1888, p. 1044) puteLumbricus intoa glass vessel 

 filled with bits of moistened blotting-paper. They gradually 

 evacuate the earthy particles from the gut, and fill it instead 

 with paper. 



VOGT and YDNG (Trait v d'Anat. Comp. Prat.j v) recom- 

 mend coffee-grounds instead of paper, as they cut better after 

 imbedding. 



JOEST (Arcl. Eiitu'iMungsmech., v, 1897, p. 425) simply 

 keeps the worms for a few days in moist linen, and finds the 

 gut empty. 



PEARL (Journ. appl. Mic. } iii, 1901, p. 680) injects alcohol 

 of 6 per cent, through the gut of narcotised worms. 



878. Chsetopoda : Fixation. Lumbricus may be anaesthetised 

 by putting the animals into water with a few drops of chloro- 

 form. PKKKIER puts them into water in a shallow dish, sets 

 up a watch-glass with chloroform in the corner of it, and 

 covers the \vlmlr. 



