METHODS FOU INVERTEBRATES. 459 



CERFONTAINE (Arch, de BioL, x, 1890, p. 327) injects inter- 

 stitially about 2 c.c. of a 1 : 500 solution of curare. 



JAQUET (Bib. Anat., iii, 1895, p. 32) kills Lumlricus in 

 extension in 1 part of nitric acid to 125 of water. 



COLLIN (Zeit. wiss. Zool, xlvi, 1888, p. 474) puts Criodrilus 

 Jacuum into a closed vessel with a little water, and hangs up 

 in it a strip of blotting-paper soaked in chloroform. KUKKN- 

 THAL (Die mik. Technik, 1885 ; Zeit. wiss. Mik., 188(5, p. 61) 

 puts Annelids into a glass cylinder filled with water to the 

 height of 10 centimetres, and then pours 70 per cent, alcohol 

 to a depth of one to two centimeters on to the water. For 

 Opheliadas he also employs O'l per cent, of chloral hydrate 

 in sea water. 



Many marine Chaatopoda may be successfully narcotised 

 (Lo BIANCO) in sea water containing 5 per cent, of alcohol, 

 or by means of the mixture 18. 



The PolycJtfeta sedentaria may sometimes be satisfactorily 

 fixed by bringing them rapidy into corrosive sublimate. 

 Cold, not hot, solutions should be taken, as heat frequently 

 shrivels up the branchiae. Eunice and Onuphis rnay be treated 

 in the same way. 



Lo BIANCO advises killing Chaetopteridae, Sternaspidaa, 

 8pirograpJtt8 f Protida. by putting them for half an hour 

 into 1 per cent, chromic acid. Some of the sedentaria may 

 be got protruded from their tubes by leaving them for some 

 hours in 0*1 per cent, chloral hydrate in sea water. 



For EISIG'S methods for Capitellidae see Fauna u. Flora 

 Golf. Neapel, xvi, 1887, p. 295. 



See also 14 (lemon juice), and the methods 20 to 26, 

 39 and 49. 



879. Blood-vessels of Annelids (KUKENTHAL, Zeit. wiss. Mik., 

 1886, p. 01). The animals should be laid open and put for 

 two or three hours into aqua regia (4 parts of nitric acid to 

 2 of hydrochloric acid). Vessels black, on a yellow ground. 



BEEGH (Anat. Heft a, xlv, 1900, p. 392, and xlix, 1900, p. 

 599) puts small Annelids for a week or more into equal parts 

 of 1 per cent, nitric acid and 1 per cent, nitrate of silver, or 

 into 50 parts of nitrate, 25 of formic acid, and 25 of water, 

 dissects out the organs and exposes to light. Marine forms 

 may be treated by HARMEK'S process. 



