460 CHAPTER XXXVI. 



830. Nerves of Anneliib. The motliylon blue method and 

 the bichromate of silver method of Golgi (the rapid method). 

 For the latter see v. LENHOSSEK (Arch. mik. Anat., xxxix, 

 p. 102). 



LANGDON (Journ. Comp. Neur., x, 1900, p. 4) injects strong 

 solution of methylen blue into the body cavity of Nereis, and 

 puts the animal for some hours into sea-water in the dark, 

 fixes the stain by Bethe's method, and makes paraffin 

 sections. 



See also M. LEWIS, Anat. Anz., xii, 1896, p. 292 ; ATHESOX, 

 ibid., xvi, 1899, p. 497 ; and the methods of APATHY -342, 

 368, 371, and 773. 



881. Hirudinea- For the methods of killing see those given 

 for Lumbricuiin 878, also 20 to 26, and 49. 



WHITMAN (Meth. in mic. Anat , p. 27) recommends that 

 they be killed with sublimate. 



I have obtained better results myself by narcotising with 

 carbonic acid ( 26), and fixing with liquid of Flemming. 

 I have also found that lemon juice kills them in a state of 

 very fair extension. 



APATHY succeeds with alcohol of 40 per cent. 



GRAF (Jen. Zeit., 1893, p. 165) has obtained good results 

 by narcotising with decoction of tobacco. 



882. Injection. WHITMAN (Amer. Natural., 1886, p. 318) 

 states that very perfect natural injections may often be 

 obtained from leeches that have been hardened in weak 

 chromic acid or other chromic liquid. 



JACQOET (Mitth. Zool. 8 tat. Neapel, 1885, p. 298), for 

 artificial injections, puts leeches into water with a very small 

 quantity of chloroform, and allows them to remain a day or 

 two iu the water before injecting them. 



883. Nervous System. Impregnation with gold. BRISTOL 

 (Journ. of MorpJi., xv, 1898, p. 17) kills in formic acid of 15 

 to 20 per cent., puts for twenty-five minutes into 1 per cent, 

 gold chloride, reduces in formic acid of 1 per cent, (twelve 

 to eighteen hours), and imbeds in paraffin. See also 342, 

 368, 371 and 773. 



884. Nephridia. SHEARER (Quart. Journ. Micr. Sci., lv, 

 1010, ]). 2<SS) stains Histriobdella intni vihnn with very weak 



