22 MEDICAL BACTERIOLOGY 



GIEMSA'S STAIN 



Azur II-eosin 3.0 Gm. 



Azur II 0.8 Gm. 



Glycerin (C.P.) 250.0 cc. 



Methyl alcohol (C.P.) 250 . o cc. 



Grind up dyes in alcohol, then add glycerin. Fix film in methyl alcohol, 

 stain 5 minutes with: 



Giemsa 14 gtt. 



H 2 O 10 gtt 



For the principles and mechanism of staining of microorganisms, consult 

 "The Microtomist's Vade-Mecum," A. B. Lee, P. Blakiston's Son & Co., 

 Philadelphia. 



