42 MEDICAL BACTERIOLOGY' 



Calcium Carbonate Bouillon. Sterilize powdered calcium carbonate or 

 marble chips in hot-air sterilizer at i5oC. for 2 hours. 



1. Plain bouillon (meat infusion), 100 cc. 



2. Calcium carbonate, i Gm. 



3. Glucose, i Gm. 



Sterilize in steam sterilizer at iooC. 20 minutes each day for 3 days. 



Dunham's Solution (Peptone -salt-sol.). 



1. Peptone (Witte's), 10 Gm. 



2. Sodium chloride, 5 Gm. 



3. Distilled water, 1000 cc. 



Boil 15 to 30 minutes, add water to make up for evaporation, filter while 

 hot and again when cold. 



Sterilize in steam sterilizer at iooC. 20 minutes each day for 3 days. 



Trypsinized Peptone-water, (Rivas). 



1. Dissolve 10 Gm. peptone (Witte's) with gentle heating in 200 to 300 

 cc. of water. 



2. Dissolve 0.5 Gm. trypsin in 20 cc. of water by shaking and gentle heating 

 (less than 4oC.). 



3. Mix and digest for i to 3 hours at 38C., stirring gently every 15 minutes. 



4. Adjust reaction to neutral, add water to make 1000 cc., boil, filter, tube 

 and sterilize in an autoclave. 



This medium is superior to Dunham's for detection of indol production. 



Ox-bile Bouillon. 



1. Plain bouillon, 100 c.c. 



2. Ox bile, 100 cc. 



Sterilize in steam sterilizer 20 minutes each day for 3 days. 



Bile Medium (Buxton and Coleman's). 



1. Ox bile, 900 cc. 



2. Glycerin, 100 cc. 



3. Peptone, 2 Gm. 



Sterilize in steam sterilizer 20 minutes each day for 3 days. 



Hiss Serum Water Medium. 



1. Beef-blood serum, 100 cc. 



2. Distilled water, 300 cc. 



3. Glucose or other sugar, 4 Gm. 



4. Sat. aqueous sol, litmus q. s. to make blue. 



Sterilize in steam sterilizer 20 minutes each day for 3 days. 



MacConkey's Bile Salt Medium. 



1. Sodium taurocholate, 0.5 Gm. 



2. Peptone, 2 Gm. 



3. Water, 100 cc. 



