124 MEDICAL BACTERIOLOGY 



clumps and that the organisms are motile. Inject the 2 cc. of emulsion into 

 the peritoneal cavity of a guinea-pig that has been immunized against the 

 cholera spirillum. Ten minutes later withdraw several drops of the peritoneal 

 fluid, place on hanging-drop slide and examine with the microscope. If the 

 organism injected into the pig was the cholera spirillum it will have lost its 

 motility and will appear as granular dots. If it was not the cholera spirillum 

 it will appear unaltered, morphology and motility the same as before injection. 

 The isolation of the cholera spirillum from water will be described in the 

 chapter devoted to the examination of water. 



