HYPHOMYCETES 163 



Aspergillus fumigatus produces a tubercular-like infection in pigeons, fowl, 

 guinea-pigs and rabbits. Growth on culture media is at first whitish, then green 

 or greenish-blue; later it becomes brown or black. 



Penicillium glaucum has been found in chronic inflammatory conditions of 

 the naso-pharynx and Eustachian tube. Growth on culture media is green. 

 This mold is used in the manufacture of certain kinds of cheese. 



Mucor mucedo is the mold most commonly observed on foodstuffs; it is 

 whitish and appears like cotton. This organism has been reported as the excit- 

 ing cause in several cases of tubercular-like infections of the lungs, in which 

 cases it was found in the sputum. 



The development of hyphae, basidia and sporulation of many of the higher 

 forms of bacteria, molds and yeasts can be studied best as follows: 



Liquefy a tube of sterile glucose agar, spread a small drop of it in a thin even 

 film on a clean slide with a sterile pipette, and when solid lightly stroke the 

 surface with a platinum loop charged with the material to be studied. Place 

 a cover glass in it and incubate at 37C. At frequent intervals place on micro- 

 scope and observe growth with % and % objectives and high-power eyepiece 

 i ox to i8x. 



Resistance. The spores of molds are frequently quite as resistant or more 

 resistant to the germicidal influence of heat, light and chemicals than the spores 

 of bacteria. It is a matter of practical importance to know that formaldehyde 

 is less germicidal to molds than to bacterial spores; that molds are especially 

 susceptible to phenol solutions, and very weak solutions of copper sulphate, 

 i : 1000 or less, will prevent their development. 



