184 MEDICAL BACTERIOLOGY 



FROZEN EGGS AND DESICCATED EGGS 



1. Weigh out i.o Gm. of egg on a sterile watch crystal, put in sterile flask 

 with 99 cc. of sterile water, shake until entirely dissolved and thoroughly mixed. 



2. Transfer i cc. of first dilution (i : 100) to a second flask containing 99 cc. 

 of sterile water and thoroughly mix, making a i : 10,000 dilution. 



3. Plant both plain agar and litmus lactose agar plates with the following 

 quantities of the second dilution: o.i cc., 0.2 cc., 0.5 cc., 0.7 cc. and i.o cc. 



4. Incubate, inspect and count as when examining eggs taken from shell. 



