2Q MICEO-ANALYTICAL METHODS 



FORM No. Ill 

 Bacteriological Examination 



(No., label, dates, condition of seals as for form I.) 



i. Direct count. (Thoma-Zeiss hemacytometer with Turck ruling.) 



1. Bacilli per cc 



2. Cocci per cc 



II. Plate and tube cultures. (Lactose-litmus-agar.) 



1. Temperature differential test. 



a. (20 C.) colonies per cc 



b. (38 C.) colonies per cc 



2. Color differential test. 



a. Pink or yellow colonies per cc 



b. Not pink or yellow colonies per cc 



3. Gelatin liquefying colonies per cc 



4. Indol reaction () 



5. Neutral red reduction () 



6. Gas (hydrogen) formula 



7. Gram stain behavior () 



8. Presumptive colon bacillus test (). 



a. Amounts used 



b. Number of tests 



c. Rating 



III. Special tests 



IV. Conclusions. 



. . .Analyst. 



