TEST FOR TYPHOID BACILLUS 



III 



typhoid germs. These conditions will enable the highly motile 

 Bacillus typhosus to reach the more remote loops first where they 

 may be taken out by means of the platinum loop or the pipette. 

 In place of the loop tubes above described and which can be 



FIG. 33. Loop tubes for culturing and isolating typhoid bacilli and other 

 motile bacteria as explained in the text, i, Single-loop or U-tube; 2, double-loop or 

 W-tube; 3, three-loop tube; 4, four-loop tube. The tubes are filled with phenol 

 broth or other desirable media and inoculated at the ends marked (a). Material 

 for subculturing and for microscopical examination is taken from the opposite end 

 (b), at varying intervals of time. 



made in the laboratory, it would be preferable to use a single tube 

 of four or five loops provided with openings at each of the upper 

 turns of the loops, thus making five or six openings in all, from 

 which the quantities to be examined and plated may be taken. 



