208 



BACTERIOLOGICAL METHODS 



filled while the containers and the syrup are still hot and then her- 

 metically sealed by means of sterilized stoppers. 



In the case of medicinal syrups and sugar-bearing medicines 

 which are contaminated by bacteria, it must be borne in mind 

 that the active constituents present are also more or less com- 

 pletely decomposed. Such substances should be quite free from 

 contamination and the presence of marked contamination should 



c - 



I 



-a 



-b 



rsi a 



-b 



FIG. 69. Stab culture appearance of B. californiensis in beef extract gelatin 

 tubes, i, Appearance of growth on third day after inoculation; 2, deep stab cul- 

 ture 24 hr. old, from tube (i); 3, same as (2) 36 hr. old. Liquefaction of gela- 

 tin is noticeable 54, same as (2) 3 days old. In the course of 2 weeks the entire 

 contents of tube became liquefied. 



form the basis for the condemnation of such products. The 

 adoption of a numerical bacterial, yeast and mold standard would 

 appear highly desirable. 



Among the products which are classed with the syrups are 

 syrup or molasses and treacle from the sugar cane, and the sorghum 

 molasses of the central and northern states, maple syrup and 

 other syrups of commerce including the so-called corn syrup which 



