197 

 Patient's serum agglutinated as follows: 



a Slight. 



The bacillus of mouse typhoid was isolated from the patient's 

 stools July 21 and 23. Negative results thereafter. Examinations 

 continued a month. 



Same organism isolated from urine July 21. Negative thereafter. 



The author concludes that the B. typhi murium is able to cause in 

 man a rather severe acute illness of short duration. 



Shibayama gives the following report of outbreaks of human 

 infection that have come to his knowledge in Japan, where mouse- 

 typhoid virus has been used in considerable quantities. 



Outbreak 1. In April, 1905, in a village of the Province of Saitama, 

 30 people became ill and 2 died with severe gastro-intestinal symp- 

 toms. Outbreak investigated by Dr. H. Sezuki, district medical 

 officer, formerly of the Tokio Institute for Infectious Diseases. 



It was found that all the 30 people had partaken of a dish of cooked 

 vegetables served at a meeting of the town council, and that for 

 application of sauce to these vegetables (after cooking) a wooden 

 vessel had been used which two days before had been used for mixing 

 mouse-typhoid virus with meal, without subsequent cleansing or 

 sterilization. 



The symptoms came on within twelve to forty-eight hours there- 

 after (usually twenty hours), chill or chilly sensations, rise of temper- 

 ature to 38 or 39 C., or even to 40 C. ; face flushed; pulse acceler- 

 ated; great weakness; thirst, nausea, colicky pains in abdomen fol- 

 lowed by severe diarrhea and vomiting. In general, the fever and 

 diarrhea lasted two or three days; but malaise, anorexia, weakness, 

 and mucous stools persisted for several days. The more severe 

 cases showed choleraic symptoms of collapse. Two persons died 

 in spite of medical treatment a 6-year old boy and a man of 43, on 

 the second and third day, respectively. 



From the intestinal contents of these two cases, from the stools of 

 several other cases, and from the remnants of the dish of vegetables 

 in the wooden bowl an organism was isolated, which was demon- 

 strated to be identical with the bacillus of mouse typhoid.* 



a Shibayama, G.: Ueber Pathogenitat der Mausetyphusbazillen f iir den Menschen. 

 Munch, med. Woch., May, 1907, p. 979. 



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