200 



The other three occurred in a family which, on the day before the 

 onset of the illness, had drunk raw milk obtained from a house where 

 the rat virus had been used shortly before, and only three members 

 of the family who drank the milk became ill. 



In order to prove the etiology of these cases Fleischanderl, who 

 had never suffered any intestinal troubles, had had nothing to do 

 with any case of typhoid fever for a year, and was in excellent health, 

 took a culture of the mouse-typhoid bacillus as used in the neighbor- 

 hood, rubbed a glass rod over the surface, washed it off in a glass of 

 water, and drank this before breakfast on the morning of May 3. 



In twenty-two hours he experienced mild, increasing body pains, 

 followed within a few hours by diarrhea, and a few hours later by 

 slight chill, rise of temperature to 38.2, pulse 106, severe pains in 

 body, and feeling of great weakness. 



May 4, 9 p. m: Temperature 39.2 C., pulse 120. Height of symp- 

 toms. 



May 5: Temperature 38.2 to 38.5 C., pulse 106 to 120. Other 

 symptoms continued. 



May 6 : Temperature and pulse normal. All symptoms disappeared 

 except weakness, which lasted two days. 



Bacteriological investigations conducted by Herbert Berger in the 

 K. K. Serotherapeutischen Institut and by Doctor Reichel, assistant 

 in the Hygienic Institute of the University of Vienna, follow: 



From the stools of one of the patients infected from milk an organ- 

 ism was isolated which, injected into mice (1 c. c. emulsion of forty- 

 eight-hour culture), killed them in two to five hours. Mice infected 

 by eating these dead mice died in thirty to forty-eight hours. 



Control mice inoculated similarly with a culture of the market 

 mouse-typhoid virus died in twenty to thirty hours, while the mice 

 infected through eating these died after three to four days. 



The following strains were used for cultural agglutination tests: 



A. From stools of patient infected from milk. 



Bl. Market virus used in injecting mice. 



B2. Market virus taken by author. 



C25. From stools of author twenty-five hours after infection. 



C55. From stools, of author fifty-five hours after infection. 



LL. Stock culture of Loeffler's mouse-typhoid bacillus. 



LP. Stock culture of para-typhoid bacillus. 



All organisms (A C55) were demonstrated as motile bacilli, not 

 liquefying gelatine, not forming lactic acid, and forming gas from 

 dextrose. 



The serum of a rabbit after two injections of LL agglutinated LL 

 and LP in dilution of 1:1280, did not agglutinate A, Bl', B2, C25, 

 and C55. 



