292 



BACTEKIOLOGY OF THE EYE 



ance, and in three cases it was possible to obtain cultures of the Streptotlirix. 

 Cultural isolation and exact definition of the mould were only successful in two of 

 these cases. In one of them the liquefied anaerobic culture was left for eighteen 

 months, when the StreptotJirix was still living, the other organisms having died 

 out. This was an undoubted Streptotlirix without any marked pathogenicity for 

 animals. In the second case successful anaerobic cultures were obtained on a 

 weakly acid medium. la the third failure was due to the excessive growth of the 

 other organisms when the medium was inoculated. In every case cultures should 

 be grown anaerobically on acid media. 



FIG. 63. 

 ANAEROBIC CUL- 

 TURE, FROM A 

 CONCRETION SENT 

 ME BY PROFESSOR 

 LANGE. 



FIG. 64. SECTION OF THE ANAEROBIC CULTURE. 



At the periphery numerous secondary colonies are developing, with 

 radiating branched processes. 



I have succeeded in obtaining another pure culture from a concretion sent me by 

 Professor O. Lange (Braunschweig), but its full description is not yet available. 

 The culture and a section of the growth are shown in Figs. 63 and 64. 



Dalen has recently succeeded in obtaining cultures from two cases. The fresh 

 concretions did not show any globular bodies, but only a tangle of fibres, which 

 branched very sparsely. Dalen only obtained the Streptotlirix in deep agar 

 stab cultures. Small nodules developed here, and could be further inoculated for 

 two to three weeks ; filaments with sparse branchings also occurred, and in old 

 cultures a free polymorphism. Subcutaneous and intraperitoneal injections were 

 negative. Dalen did not attempt any further classification of the variety of 

 Streptothrix present. 



Recently the question of whether these are Leptotliricece has again been brought 

 forward a return to the views of A. von Graefe's time. Hirschberg described 



