CONTENTS. 



CHAPTER I. 

 Microscopical and Physiological Examination. 



PAGES 



1. Microscopical Preparations ; Staining, and Microscopical Examination, 1-10 



2. Biological Research ; the Microscope ; Moist Chambers, . . . 10-13 



3. Sterilisation 13-31 



(a) Sterilisation of Glass and Metal Articles, .... 14-15 



(b) Sterilisation of Liquids and Solid Nutritive Substrata, . . 15-31 



4. Disinfection in Practice, 31-36 



5. Flasks 36-40 



6. Nutritive Substrata, . 40-43 



7. Preparation of Pure Culture, 43-55 



(a) Physiological Methods, 45-47 



(b) Dilution Methods, 47-55 



8. Counting the Yeast Cells, 55-58 



CHAPTER II. 



Biological Examination of Air and Water. 



Introduction, ............ 59-60 



Air Analyses 



Miquel's Methods and Results, 60-62 



Methods of Hesse, Hueppe, von Schlen, Frankland, Miquel, Petri, 



andFicker, 62-63 



Sampling Air, ........... 64 



Miquel's Criticism of the Gelatine Process, ..... 64 



E. C. Hanson's Researches on Air, . . . . . . 65-69 



Saito's Researches, 69 



Water Analyses 



Holm's and Jorgensen's Examination of Water, .... 69-71 



Examination of Ice, 71 



Methods of Hansen, Wichmann, Lindner, and Jorgensen, . . 71-75 



