SACCH. PASTORIANUS II. 



347 



Film-formation : 



At 34 C. no film-formation occurs. 

 26-28 feebly-developed film-specks 



are seen after 



7-10 days. 



20-22 



13-15 



6- 7 



3- 5 



. 69.) 



,, ,, 1- 2 months. 



5- 6 



like Fig. 69, but without the large colonies. 

 2- 3 no film-formation occurs. 

 Microscopic appearance of the cells in the films : 

 At 20 to 28 C. almost the same forms occur as in the 

 sedimentary yeast. 



At 13 to 15 C. strongly-developed mycelial colonies of 



very elongated, sausage-shaped cells are fairly frequent (Fig. 69). 



In old cultures of films the cells are smaller than in the 



sediment ; very irregular and sometimes almost thread-like 



cells are found. 



Saccharomyces intermedius or Saccharomyces Pastorianus II. 



Hansen. (Figs. 70, 71.) 

 Feeble top-fermentation yeast. 



Fig. 70.Saccharomycfs Pastorianus II. (Hansen).- Cell-forms of young sedimentary yeast 



(after Hansen). 



Sedimentary forms grown in wort : Mainly elongated, 

 sausage-shaped cells, but also large and small, oval, and round 

 cells (Fig. 70). 



