352 



MICRO-ORGANISMS AND FERMENTATION. 



Ascospore-formation (Fig. 58, 5) : 



At 32-5 C. no ascospores are developed. 

 30-5-31-5 the first indications are seen after 36 hours. 



29-5 



25 



18 



15 



10-5 



7-5 



4 



23 



^1 

 33 



45 



4 



days, 



no ascospores are developed. 

 Size of the spores 2 to 4 /u. 



Film-formation : 



At 38 C. no film-formation occurs. 



feebly-developed film-specks 



are seen after . 8-12 days. 

 9-16 

 10-17 



15-30 (Fig. 75.) 

 ,, ,, 2- 3 months, 



no film-formation occurs. 



33-34 



26-28 



20-22 



13-15 



6- 7 



5 



Microscopic appearance of 

 the cells in the films c 



At 20 to 34 C. and 6 to 

 7 C., the cells are smaller and 

 more sausage-shaped than in 

 the sedimentary yeast. 



At 13 to 15 C., freely- 

 branched and strongly- 

 developed colonies of long 

 or short sausage - shaped 

 cells, often with verticillated 

 branches (Fig. 75). 



In old cultures of films, 

 the cell forms are the same 

 as at 13 to 15 C. 

 Streak cultures of this species in wort-gelatine (wort with 

 the addition of about 5-5 per cent, of gelatine), in the course 



Fig. 74. Saccharomyees ettipcoideus I. (Han- 

 sen). Cell-forms of young sedimentary 

 yeast (after Hansen). 



