92 MICROSCOPICAL DIAGNOSIS. 



THE DIFFERENTIAL STAINING OF NUCLEATED 

 BLOOD CORPUSCLES. 



T 



HE fluids used for this purpose are two, which are designated 

 as A and B. Their formulas are as follows : 



A. 



Eosin, 5 grains. 

 Distilled water, 4 drachms. 

 Alcohol, 4 drachms. 

 Dissolve the eosin in the water and add the alcohol. 



B. 



Methyl analin green, 5 grains. 

 Distilled water, r ounce. 



The blood should be spread upon the slide, by placing a drop 

 upon one end and quickly drawing the smooth edge of another slide 

 over it. This, if well done, will leave a single layer of corpuscles 

 evenly spread over the central part of the slide. 



When the corpuscles on the slide are thoroughly dry, which will 

 only require a few minutes, the slide should be 'flooded' with stain 

 A. 



This should be allowed to remain on for about three minutes, 

 at the end of which time it may be washed by gently waving back 

 and forth in a glass of clean water. Before it is allowed to dry, the 

 corpuscles should again be flooded, this time with stain B. After 

 two minutes exposure to this fluid, the slide should be washed, as 

 before, and set away to dry. When dry, a drop of Canada balsam 

 may be put upon the blood, a cover-glass applied, and the whole 

 gently warmed until the balsam spreads out properly. When hard 

 it may be finished the same as is usual with balsam mounts. 



If now examined with the midroscope, the corpuscles will be 

 found to be well stained with red, while the nuclei and "leucocytes" 

 will be a blueish-green. 



The granular appearance, which is ordinarily seen in the nuclei, 

 now shows with a vigor and sharpness which is difficult of 'descrip- 

 tion, while the whole corpuscle is as brilliant as a newly cut ruby. 



This method was originally given by Prof. A. Y. Moore, and it 

 first appeared in the August number of u The Microscope" for 1882. 



