MODERN METHODS OF EXAMINATION. 69 



3. STAINING FLUIDS. 



Thier setts Carmine Fluids. 

 a. RED FLUID. 



1. Carmine, 1 part. 



Caustic ammonia, . . . . . 1 " 



Distilled water, 3 parts. Filter. 



2. Oxalic acid, 1 part. 



Distilled water, 22 parts. 



1 part of carmine solution to 8 parts of the acid solution, add 12 parts 

 absolute alcohol. Filter. After staining wash in 80 per cent, alcohol. 



b. LILAC FLUID. 



Borax, . . ..: 4 parts. 



Distilled water, 56 " 



Dissolve and add, 



Carmine, . . . . . . .1 part. 



Mix with twice the volume of absolute alcohol and filter. 



Beetle's Carmine Fluids. 



Carmine, ....... 10 grains. 



Strong liquor ammonia, drachm. 



Glycerin, ....... 2 ounces. 



Distilled water, . . . . 2 " 



Alcohol, ounce. 



Dissolve the carmine in the ammonia in a test-tube by aid of heat ; boil 

 it and cool and add the other ingredients. Filter. 



Acid Carmine Fluid. Mix ammoniacal solution of car- 

 mine with acetic acid in excess and filter. This is said 

 to stain diffusely, but adding glycerin with muriatic acid 

 (1 : 200), concentrates the color in the cell-nucleus. 



Anilin (or Magenta] Red Fluid. 



Fuchsin (crystal), 1 centigramme. 



Absolute alcohol, .... 20-25 drops. 

 Distilled water, ..... 15 cubic centim. 



Anilin Blue Fluid. Anilin blue, treated with sulphuric 

 acid and dissolved in water till a deep cobalt color is 

 obtained. 



