PREFACE TO EIGHTH EDITION. 





IN the preparation of this new edition of Dr. Bolles Lee's well- 

 known book I have received the assistance of Professor W. M. 

 Bayliss, Dr. C. Da Fano, Dr. A. Drew, Dr. W.. Cramer, and Mr. 

 J. Thornton Carter. It has been almost entirely due to these 

 workers that my plans for the new edition have been able to be 

 carried out in the way I wished. Thanks to them, this book may 

 be considered a most complete and stimulating book of reference for 

 the research worker. 



Professor W. M. Bayliss, to whom I am especially grateful, has 

 rewritten Chapter XI. on " Staining." Dr. C. Da Fano has revised, 

 and in some cases almost completely rewritten, the difficult chapters 

 on " Neurological Techniques," which constitute indeed a special 

 branch of microtomy. Dr. A. Drew has completely rewritten the 

 valuable section on " Protozoa " ; while from Dr. W. Cramer's pen 

 has come most of the article on " Fatty Substances," which will 

 bring to the notice of embryologists and histologists the newest 

 advances in the micro-chemistry of this subject. Mr. J. Thornton 

 Carter has revised the section on " Teeth and Bone." I have 

 personally been responsible for the rest of the book, besides having 

 written special chapters or sections on " Chromatin, Chromosomes, 

 Nucleoli, Glycogen, Iron, Yolk, Fat, Mitochondria, Golgi Apparatus 

 and Benzidine Dyes " ; I have completely rewritten the section on 

 "Mammalian Embryological Methods." In the sections dealing 

 with the cytoplasmic inclusions, most of the various structures, 

 known as "attraction sphere rodlets," "idiozome," " Golgi-Kopsch 

 apparat," " nebenkern batonettes," etc., are grouped under the 

 term " Golgi apparatus," as now seems justifiable in view of the 

 results of modern researches on the subject. 



A small, yet very important, addition to the present volume has 

 been the inclusion of two new methods for staining bacteria in tissue 

 ( 089, 761). The histologist and cytologist are often puzzled to 

 know whether certain enigmatic bodies they find are or are not 

 bacterial in nature. 



