CHAPTER V. 63 



111. Piero-platinic Formol (M. and P. BOUIN, Bibl. Anal, 1898, 

 f. 2, p. 2). 



Platinum chloride, 1 per cent. sol. . . 20 parts. 

 Picric acid, saturated sol. . . . 20 



Formol 10 



Formic or acetic acid . . . 5 



The mixture does not keep more than a day or two, and it is probably 

 inferior. 



BOUIN also (Arch. Biol., xvii, 1900, p. 211) simply substitutes forinol 

 for the osmic acid in HERMANN'S mixture, 45. 



112. Sublimate Formol (M. and P. BOUIN, loc. cit.). A similar 

 mixture, in which sublimate of 1 per cent, is substituted for the platinum 

 chloride. 



Another formula of the same authors (Arch. Biol., xvii, 1900, p. 211) 

 is 1 part of formol to 3 of saturated aqueous sublimate. Rinse with 

 water and bring into alcohol of 70 per cent. 



SPULER (Encycl. mik. TecJmik., 1st ed., p. 1280) adds to sublimate of 

 3 per cent, or more 1 per cent, of glacial acetic acid and 10 per cent, of 

 formol. 



MANN (Verh. Anat. Ges., 1898, p. 39) takes for nerve-cells 2| grms. 

 sublimate, 1 grm. picric acid, 5 c.c. formol, and 100 c.c. water, or (Methods, 

 etc., p. 97) for all tissues 2| grms. sublimate, 20 c.c. formol, and 80 c.c. 

 water. 



BRANCA (Journ. Anat. et Phys., xxxv, 1899, p. 767) adds 10 parts of 

 formol and 1 of acetic acid to 60 parts of saturated solution of picric 

 acid in saturated aqueous sublimate. 



NOWAK (Anat. Anz., xx, 1901, p. 244) takes 30 parts of saturated 

 sublimate, 30 of 1 per cent, chromic acid, 27 of water, 3 of acetic acid; 

 and 10 of formalin. 



113. Formol-Muller. This is the name given by OBTH (Bed. 

 klin. Wochenschr., 1896, No. 13) to a mixture of 1 part of formol 

 with 10 of liquid of Miiller ( 53). It should be freshly made up. 

 Fix for three hours in the stove, or twelve at normal temperature, 

 wash out with running water. Much used, especially for nervous 

 tissues. 



MOELLER (Zeit. wiss. ZooL, Ixvi, 1899, p. 85) takes 1 vol. of formol 

 and 4 of 3 per cent, bichromate (for the intestine of mammals). 



HELD (Abk. Sachs. Ges. Wiss., xxxi, 1909, p. 196) takes 3 per cent, 

 sol. of bichromate with 4 per cent, of formol and 5 per cent, of 

 acetic acid (for inner ear). See also MOREL and BASSAL, Journ. 

 Anat. Phys., xlv, 1909, p. 632, and HELLY and MAXIMOW formulae. 

 Look up section on " Mitochondria," especially paragraphs on 

 REGAUD and SOHEIDDE, 685 to 687. 



114. Chromic Acid Formol. Lo BIANCO fixes marine animals for half 

 to one hour in 10 parts of 1 per cent, chromic acid with 1 of forinol and 

 9 of sea-water, and passes into graded alcohols. 



