CHAPTER X. 115 



water, coats slides with it and dries them. He flattens sections on water 

 at 40 C., lifts them out on a prepared slide, and dries for five minutes 

 at 35 C. 



189. Garlic-water. HOLLANDS (Arch. d'Anat. Micr., xiii, 1911, p. 

 171) gives the following as more adhesive than albumen : 50 grms. of 

 crushed and chopped garlic are rubbed up with 80 c.c. of chloroform - 

 water (Codex, A.C.) and filtered after twenty-four hours. Use as 

 albumen. 



190. SCHALLIBAUM'S Collodion (Arch. mikr. Anat., xxii, 1883, p. 565). 

 One part of collodion shaken up with 3 4 parts of clove or lavender 

 oil. Use as albumen. Sections can be treated with alcohol (not 

 absolute) and divers staining fluids. I do not find it safe for this. 

 KABL, however (Zeit. wiss. Mik., xi, 1894, p. 170), finds that it is if you 

 take 2 parts of collodion to 3 of clove oil, and make up fresh every four 

 or five days. 



191. OBREGIA'S Method for Paraffin or Celloidin Sections (Neuro- 

 logisches Centralb., ix, 1890, p. 295 ; GULLAND, Journ. of Path., 

 February, 1893). Slides, or glass plates of any size, are coated with 

 a solution made of 



Syrupy solution of powdered candy-sugar made 



with boiling distilled water . . . .30 c.c. 

 95 per cent, alcohol . . . . 20 ,, 



Transparent syrupy solution of pure dextrin made 



by boiling with distilled water . 10 



They are dried slowly for two or three days until the surface is 

 just sticky to the moist finger. Paraffin sections are arranged and 

 heated for a few minutes to a temperature slightly above the melting- 

 point of the paraffin. The paraffin is removed by some solvent, and 

 this in turn by absolute alcohol. The alcohol is poured off, and the 

 sections are covered with solution of celloidin. The plates are left 

 to evaporate for ten minutes in a horizontal position, then brought 

 into water, in which the sheet of celloidin with the sections soon 

 becomes detached, and may be further treated as desired, e.g., as in 

 Weigert's process, 198. The evaporation must not be artificially 

 hastened. 



DIMMER (Zeit. wiss. Mik., xvi, 1899, p. 44) coats the slides with a 

 solution of about 16 parts of gelatin in 300 of warm water, and dries 

 them (two days), and proceeds in other respects as above. 



A good method for large sections, equally applicable to paraffin 

 sections, to celloidin sections, and to sections of material that has 

 not been imbedded at all. 



For BLOCHMAN'S modification of Weigert's process, by means of 

 which large sections can be preserved unmounted, see Zeit. u 

 xiv, 1897, p. 189. g_ 2 



