116 SERIAL SECTION MOUNTING. 



192. STRASSER'S Collodion Paper Method (ibid., iii, 1886, p. 346). 

 This is an extremely complicated modification of Weigert's method for 

 celloidin sections, and is only adapted for use with STRASSER'S automatic 

 ribbon-microtome. See Zeit. wiss. Mik., iii, 1886, p. 346 ; vi, 1889, 

 p. 154 ; vii, 1890, pp. 290 and 304 ; ix, 1892, p. 8 ; xii, 1895, p. 154 ; 

 and xiv, 1897, p. 39 ; also SCHOENEMANN, ibid., xix, 1903, p. 333 ; 

 STRASSER, ibid., p. 337 ; and RUPPRICHT, ibid., xxviii, 1912, p. 281. 



Methods for Watery Sections. 



193. FOL'S Gelatin (For,, Lehrb., p. 132). Four grammes of 

 gelatin are dissolved in 20 c.c. of glacial acetic acid by heating on a 

 water-bath and agitation. To 5 c.c. of the solution add 70 c.c. of 

 70 per cent, alcohol and 1 to 2 c.c. of 5 per cent, aqueous solution of 

 chrome-alum. Pour the mixture on to the slide and allow it to dry. 

 In a few hours the gelatin passes into the insoluble state. It retains, 

 however, the property of swelling and becoming somewhat sticky in 

 presence of water. The slide may then be immersed in water 

 containing the sections ; these can be slid into their places, and the 

 whole lifted out ; the sections will be found to be fixed. 



This method is specially intended for sections made under water, 

 large celloidin sections amongst others. 



Similarly, KUPPRICHT, loc. cit., last , with the needless com- 

 plication of a seriation on Strasser's collodionised paper. 



STRASSER (loc. cit., last ) also employs a dry gelatin film which 

 he makes sticky by means of carbol-xylol. 



Methods for Celloidin Sections. 



194. The Albumen Method. I find that celloidin sections may 

 be mounted on Mayer's albumen, and have the celloidin removed, 

 if desired, by putting them into ether-alcohol. Care must be taken 

 to press them down very thoroughly on to the albumen ; and it is 

 well not to have them too wet. 



Similarly, JORDAN (Zeit. wiss. Mik., xv, 1898, p. 54), and ARGU- 

 TINSKY (ibid., xvii, 1900, p. 37). See also JORDAN, ibid., 192 194 ; 

 DANTSCHAKOFF, ibid., xxv, 1908, p. 35 ; MAXIMO w, ibid., xxvi, 

 1909, p. 184 ; ANITSCHKOW, ibid., xxvii, 1910, p. 68 ; WEBER, ibid., 

 xxix, 1912, p. 186 ; EUBASCHKIN, Anat. Anz., xxxi, 1907, p. 30. 

 Weber paints over the series on the albumen with a layer of thin 

 collodion, and puts into alcohol of 50 per cent., then into a mixture 

 of equal parts of chloroform and absolute alcohol. After staining, 

 pure absolute alcohol must be avoided. 



195. SUMMERS' Ether Method (Amer. Mon. Mic. Journ., 1887, 

 p. 73). Place the sections in 95 per cent, alcohol for a minute or 



