CHAPTER XI1L 155 



256. Ehrlich's Acid Hsematoxylin (Zeit. wiss. Mik., 1886, p. 150). 

 Water 100 c.c., absolute alcohol 100, glycerin 100, glacial acetic acid 

 10, haematoxylin 2 grms., alum in excess. 



Let the mixture ripen in the light (with occasional admission of 

 air) until it acquires a dark red colour. It will then keep, with 

 constant power, for years, if kept in a well-stoppered bottle. It is 

 very appropriate for staining in bulk, as overstaining does not 

 occur. I find it excellent. 



MANN (ibid., xi, 1895, p. 487) makes up this stain with an equal 

 quantity of hsematein instead of haematoxylin. 



MAYER (Grundziige, LEE and MAYER, 1st ed., p. 154) finds 

 that this is too much and makes the mixture overstain ; 0-4 grm. 

 of hsematein is quite enough. 



For proper method of using EHRLICH'S hsematoxylin, see SCOTT, 

 669. 



257. BURCHARDT'S Pyroligneous Acid Haematoxylin (Arch. mik. 

 Anat., liii, 1898, p. 232) would seem to be superfluous at least. 



258. UNNA'S Oxidised Haematoxylin (from MARTINOTTI, Zeit. wiss. 

 Mik., xxvii, 1910, p. 31). Haematoxylin 0-5, alum 2, water 60, 

 alcohol 10, glycerin 20, peroxide of hydrogen solution 10, carbonate 

 of soda 0-05. 



MARTINOTTI, loc. cit., makes it up with hcemaiein (0-2 grm.). 



259. APATHY'S Hsematein Mixture I A (Mitth. Zool. Stat. Neapel, 

 xii, 1897, p. 712). Make (A) a solution of 9 per cent, alum, 3 per 

 cent, glacial acetic acid, and 0-1 percent, salicylic acid in water, 

 and (B) a 1 per cent, solution of hsematoxylin in 70 per cent, alcohol, 

 preserved for six to eight weeks in a bottle not quite full. Mix 

 1 part of A with 1 of B and 1 of glycerin. Stains either sections or 

 material in bulk. Apathy uses it for staining neuro-fibrils. 



260. KLEINENBERG'S Haematoxylin (Quart. Journ. Micr. Sci., Ixxiv, 

 1879, p. 208). Highly irrational and very inconstant in its composition 

 and its effects ; see early editions ; also the criticism of MAYER (Mitth. 

 Zool. Stiit. Neapel, x, 1891, p. 174), and that of SQUIRE in his Methods 

 and Formulae, p. 25, and the alternative formulae of SQUIRE (loc. cit.) 

 and of VON WISTINGHAUSEN (Mitth. Zool. Stat. Neapel, x, 1891, p. 41). 



261. MAYER'S Haemacalcium (Mitth. Zool. Stat. Neapel, x, 1891, 

 p. 182). Hsematein (or hsemateate of ammonia, 238, 239), 

 1 grm. ; chloride of aluminium, 1 grm. ; chloride of calcium, 50 grins. ; 

 glacial acetic acid, 10 c.c. (or common acetic acid, 20 c.c.) ; 70 per 

 cent, alcohol, 600 c.c. Rub up finely together the first two ingre- 

 dients, add the acid and alcohol, dissolve either cold or with heat ; 

 lastlv add the chloride of calcium. 



