CHAPTER XIII. 157 



cent, solution of hsematoxylin in 70 or 80 per cent, alcohol. Differentiate 

 sections of 10 to 15 p., half the time of staining, sections of 25 to 40 ^ 

 twice the time of staining, in 1 per cent, solution of bichromate of 

 potash in 70 to 80 per cent, alcohol, and wash out in alcohol of 70 per 

 cent. All these processes should be done in the dark. 



For celloidin series of sections, Apathy (ibid., 1889, p. 170) stains in 

 the hsematoxylin solution as above for ten minutes ; then removes the 

 excess of heematoxylin fluid from the sections by means of blotting- 

 paper, and brings the series for five to ten minutes into 70 per cent, 

 alcohol containing only a few drops of a strong (5 per cent.) solution of 

 bichromate. 



287. SCHULTZE'S Chrome Haematoxylin (Zeit. wiss. Mik., xxi, 1904, 

 p. 5). The tissues to be fixed for twelve or more hours in a bichromate 

 or chromic acid solution, 'preferably an osmium -bichromate mixture or 

 liquid of Flemming, then to be washed out for twenty-four hours in 

 50 per cent, alcohol in the dark and stained for twenty-four hours or 

 more in 0-5 per cent, hsematoxylin in alcohol of 70 per cent., then 

 washed out in alcohol of 80 per cent. 



268. HANSEN'S Chrome Hsematoxylin (ibid., xxii, 1905, p. 64). 

 Ten grms. of chrome alum boiled in 250 c.c. of water till green, and 

 1 grm. hsematoxylin (dissolved in 15 c.c. of water) added ; to the 

 mixture when cold add 5 c.c. of sulphuric acid of 10 per cent, and (drop 

 by drop) a solution of 0-55 grm. of bichromate of potash in 20 c.c. of 

 water. Filter before use. Wash out with water free from air. 



269. Vanadium Haematoxylin (HEIDENHAIN, Eneyclop. mik. Technik., 

 1903, p. 518). Add 60 c.c. of a 6 per cent, solution of haematoxylin 

 to a 0-25 per cent, solution of vanadate of ammonium (quantity not 

 stated ; should be 30 c.c., see COHN in Anat. Hefte, xv, 1895, p. 302). 

 The mixture to be used after three or four days ; it will not keep over 

 eight days. To be used with sections of sublimate material. A strong 

 plasma stain for special purposes, especially mucus glands. 



270. BENDA'S Copper Haematoxylin (Arch. mik. Anat., xxx, 1887, 

 p. 49). See fourth edition. According to my experience, not to be 

 compared with iron hsematoxylin, and superfluous. 



271. MALLORY'S Phospho-molybdic Acid Hsematoxylin (Anat. 

 Am., 189i, p. 375). One part 10 per cent, phospho-molybdic acid 

 solution, 1 part h&ematoxylin, 100 parts water, and 6 to 10 parts 

 chloral hydrate. Let the solution ripen for a week in sunlight, and 

 filter. Chiefly for central nervous system. Sections should be 

 stained for from ten minutes to one hour, and washed out in two or 

 three changes of 40 to 50 per cent, alcohol. It is necessary that the 

 solution should be saturated with haematoxylin in order to obtain 

 the best results ; if a good stain be not obtained at once, more 

 hsematoxylin must be added. Water must never be used for 

 diluting it. 



