158 HMMATEIN (HMMATOXYLIN) STAINS. 



See also KIBBERT (Centralb. allg. Path., vii, 1896, p. 427 ; Zeit. wiss. 

 Mik., xy, 1898, p. 93), PATELLANI ( Mon. Zool. Ital., xiii, 1902, p. 6), and 

 GOLOVIN (Zeit. wiss. Mik., xix, 1902, p. 184). 



SARGENT (Anat. Anz., xv, 1898, p. 214) quotes this stain, preceded by 

 mordanting for twenty-four hours in 5 per cent, sulphate of copper, as 

 KEN TON'S. 



KODIS (Arch. mik. Anat., lix, 1901, p. 211) takes hsematoxylin, 1 part ; 

 molybdic anhydride, 1-5 ; water, 100 ; H 2 2 , 0-5, or a crystal of HgO. 



POLICE (Arch. Zool. Napoli, iv, 1909, p. 300) takes 0-35 grm. hsema- 

 toxylin, 10 drops phospho -molybdic acid of 10 per cent., 10 grms. chloral 

 hydrate, and 100 grms. alcohol of 70 per cent. 



272. MALLORY'S Phospho-tungstic Hsematoxylin (Journ. Exp. 

 Med., v, 1900, p. 19 ; Zeit. wiss. Mik., xviii, 1901, p. 178) : 



Hsematoxylin . . . . . .0-1 



Water 80-0 



10 per cent, solution of (MERCK'S) phospho- 

 tungstic acid . . . . . . 20-0 



Peroxide of hydrogen (U.S. Ph.) . . . 0-2 

 (Dissolve the hsematoxylin, add the acid, then the peroxide.) Stain 

 sections two to twenty-four hours, wash out with water. A poly- 

 chromic stain, nuclei blue, intercellular substances pink. I consider 

 this a fine stain. 



273. DONAGGIO'S Tin Hsematoxylin (Ann. Nevrol. Napoli, xxii, 1904, 

 p. 192). A 1 per cent, solution of hsematoxylin is poured slowly into 

 an equal volume of 20 per cent, solution of pink-salt (ammonio -chloride 

 of tin). Keep in the dark. 



274. Osmium Hsematoxylin. SCHULTZE (Zeit. wiss. Mik., xxvii, 

 1910, p. 465) treats tissues for twenty-four hours or more with osmic 

 acid of 1 per cent., washes well with water, and puts for a couple of 

 days into ripened 0-5 per cent, solution of haematoxylin in alcohol of 35 

 to 50 per cent. Wash out for a day or more with alcohol of 70 per cent. 

 Intense plasma stain. 



