170 NUCLEAR STAINS WITH COAL-TAR DYES. 



KAISER (Biblioth. Zool, H. 7, 1 Halft, 1891 ; Zeit. wiss. Mik., viii, 

 1891, p. 363) stains for forty-eight hours, and at a temperature of 60 C. 

 in saturated solution of Bismarck brown in 60 per cent, alcohol (the 

 solution to be made in boiling alcohol), and washes out (until all is 

 decoloured except the karyokinetic figures) in 60 per cent, alcohol, 

 containing 2 per cent, hydrochloric acid or 3 per cent, acetic acid. 



Methyl Violet. See ante, 278. 



Benzoazurin (MARTIN, Zeit. wiss. Mik., vi, 3, 1889, p. 193). Stain for 

 an hour or so in dilute aqueous solution, and wash out with HC1 alcohol. 



Methylen Blue. 



Nigrosin (ERRERA, Proc.-Verb. Soc. Beige de Mik., 1881, p. 134) gives 

 a good stain which resists alcohol well. 



Methyl Green is sometimes useful in certain mixtures (see next 

 chapter). 



