CHAPTER XV. 177 



clears with xylol, and mounts in xylol-balsam. Connective-tissue 

 red, elastin and all other elements yellow. 



WEIGERT (Zeit. wiss. Mik., 1904, p. 3) adds 10 parts of 1 per cent. 

 Saurefuchsin to 100 of saturated picric acid. 



See also MOLLER, op. cit., xv, 1898, p. 172. 



This stain is generally used as a contrast stain to follow hsemato- 

 xylin. APATHY (Behrens' Tabellen, 3rd ed., p. 129) takes for this 

 purpose 1 grm. of Saurefuchsin in 500 c.c. of saturated solution of 

 picrate of ammonia. 



WILHELMI (Fauna Flora Golf. Neapel, xxii, 1909, p. 18) takes 

 0-2 grm. Saurefuchsin, 0-8 grm. picrate of ammonia, 10 grin, absolute 

 alcohol, and 89 grm. water. 



E. and T. SAVINI (Zeit. wiss. Mik., xxvi, 1909, p. 3t) use a formula 

 due to BENDA. Ninety-five volumes of saturated solution of picrate 

 of ammonia are mixed with 5 volumes of 1 per cent, solution of 

 Saurefuchsin. For use, two to four drops of saturated solution of 

 picric acid are added to 10 c.c. of the mixture. This neither over- 

 stains nor attacks the primary stain. 



300. FLEMMING'S Orange Method (Arch. mik. Anat., xxxvii, 1891, 

 pp. 249 and 685). Stain sections of Flemming or Hermann material in 

 strong alcoholic safranin solution diluted with anilin water ( 286) ; 

 differentiate in absolute alcohol, containing at most (M per cent, of 

 hydrochloric acid, until hardly any more colour comes away ; stain for 

 one to three hours in gentian violet ( 287) ; wash for a short time in 

 distilled water ; treat with concentrated, or at least fairly strong, 

 aqueous solution of orange G. After at most a few minutes, whilst 

 pale violet clouds are still being given off from the sections on agitation, 

 bring them into absolute alcohol until hardly any more colour comes 

 away, clear in clove or bergamot oil, and mount in damar or balsam 

 before the last pale clouds of colour have ceased to come away. The 

 orange must be orange G. 



WINIWARTER and SAINMONT (Zeit. wiss. Mik., xxv, 1908, p. 157, 

 and Arch. Biol., xxiv, 1909, p. 15) stain for twenty-four hours in the 

 gentian, wash out after the orange for two to three hours in 100 c.c. 

 absolute alcohol with 3 to 4 drops of HC1, and differentiate finally with 

 oil of cloves. 



This is not a triple stain in the sense of giving three different colours 

 in the result ; it is a nuclear and plasmatic stain in mixed tones ; the 

 orange, apparently, combines with the gentian to form a "neutral" 

 dye, soluble in excess of the orange (203) which thus differentiates the 

 stain. 



See also FLEMMING in Arch. Anat. Phys. Anat. Abth., 1897, p. 175. 



Never popular, this clumsy and uncertain process is now little used. 



301. REINKE'S Orange Method (Arch. mik. Anat., xliv, 2, 1894, p. 262), 

 To a concentrated aqueous solution of gentian violet are added " a few 

 props " of a like solution of orange G. The solution precipitates in part. 



M. 



